The Research On Bamboo Lignin Degradation And Efficiency Composite Construction Of Strains In The Fermentation Process

China is abundant bamboo resources, bamboo fiber with green, good moisture absorption and desorption, natural anti-bacterial and anti-UV properties, has great market potential and added value.Bamboo fiber lignin seriously affected the bamboo fiber industry development process, and microbial degradation of lignin is the most effective way to solve this problem. From bamboo lignin degradation microbes needed to start, easy to use microbial culture, breeding fast, strong ability to degrade the characteristics of the chemical composition of different varieties of bamboo analysis, screening lignin degradation complex bacteria, on the basis of its degradation lignin conditions were studied to develop the future of microbial bamboo fiber material will lay a solid foundation.The main results obtained were an follows:1. Study the chemical composition of different varieties of vegetablesThrough different bamboo species, bamboo varieties of the same species of different ages and in different parts of bamboo ash, lignin, cellulose and pectin content mechanized and other important chemical analysis, due to the chemical composition of near the site of the Bambusa textilis the ash content was 2.07%, the lignin content of 22.54%,76.86%Fully cellulose content, pectin content of 1.11%; Phyllostachys pubescens chemical composition of the ash content of 1.12%, the lignin content of 21.49%, mechanized cellulose content of 77.65%,0.92% pectin content, so choose Bambusa textilis or Phyllostachys pubescens near the site of a high bamboo pulp as raw material utilization, and can reduce production costs, reduce environmental pollution, improve bamboo continuing operations and economic advantages.2. Construction of bamboo lignin degradation complex bacteriaIn antagonism between different strains, different single strain of bamboo lignin degradation elected as the index does not exist antagonist screening and high lignin degradation ability strain CN-12, HY-31, YY-36, ZYS-19; using different combinations of compound bacteria biomass, bamboo weightlessness, bamboo lignin degradation, degradation bamboo cellulose degradation selectivity coefficient index rescreening high degradation, high selectivity bamboo lignin complex combination of strains HY-YY-ZYS, and stability studies through a combination of complex bacteria, indicating that the strain complex combination of lignin degradation ability to stabilize the average degradation rate reached 38.54 percent, respectively, than the ability to degrade the strongest single strain of the degradation rate increased 8.02%.3. Bamboo lignin degradation by solid state fermentation medium optimizationIn the degradation rate and selectivity coefficient for the main index, single factor binding uniform design optimization of composite strain combination (HY-YY-ZYS) solid bamboo lignin degradation fermentation medium were optimized:ammonium tartrate 0.2 g /L glucose 14 g/L, Ca2+ 0.8 mmol/L, Mn2+ 0.6 mmol/L, the degradation rate of 46.29%, selectivity coefficient of 10.72.4. Solid bamboo lignin degradation fermentation optimization of culture conditionsIn the degradation rate and selectivity coefficient for the main index, using orthogonal design, composite strain combination (HY-YY-ZYS) solid fermentation degrade lignin bamboo culture conditions were optimized:inoculation amount was 6%, a water content of 50% temperature 32℃, pH 5.0, in this condition solid fermentation time 25d, bamboo lignin degradation rate of 60.29%, selectivity coefficient of 12.79, the degradation rate of increase of 21.75%, an increase of 9.15 choose coefficient, degradation shortened 5d, bamboo lignin degradation rate, selectivity coefficient, production efficiency have been improved, and reduce production costs.