| Antibiotics are widely used in clinical treatment, aquaculture and animal husbandry.Tetracycline(TC) and sulfamethoxazole(SMZ) are widely used in domestic. The abuse of antibiotics, which makes most of the antibiotics without human or animal fully absorbed, directly discharged into the water environment, the kind of drug in the body is difficult to be degraded, can be accumulated through the food chain. Antibiotics Bacteria Resistant(ARB) and Antibiotics Resistant Genes(ARGs) can cause serious threat to human health and ecosystem. Antibiotics,ARB and ARGs in the wastewater treatment system are more concentrate. Anaerobic anoxic aerobic(AAO) process is a traditional and widely used wastewater treatment method, the operation technology is mature. Forward Osmosis(FO) is a new technology for the separation of green membrane, which is not required to be a separate driving force. It is one of the hot spots in the world membrane separation. Therefore, it is necessary to study the removal and distribution characteristics of antibiotics, drug resistance and drug resistance genes in AAO system, and then select the AAO+FO combined process to deal with the depth of antibiotics and drug resistance. To provide a theoretical reference for the influence of antibiotics on the environment and its removal.Fate of Antibiotics and ARB during the AAO system was investigated under different sludge retention time(SRT)(5, 10, 15, 20 d). Under optimum operating condition, metagenomic technology and fluorescence quantitive PCR were applied to determine the bacterial community structure and TRGs, respectively. SRT was prolonged, and the removal rate of antibioticsincreased. Bacteria and Bacteroidetes for Bacteroides bacteria Proteobacteria deformation in AAO1 system, Shannon index size, anoxic >aerobic >anaerobic. In AAO2 system, the dominant bacteria are Proteobacteria Proteobacteria, Shannon index size, anoxic> aerobic >anaerobic. In AAO3 system, the dominant bacteria are Bacteroide Proteobacteria, Shannon index size:anaerobic> anoxic> aerobic. 3 sets of systems in the pool of 5 ARGs concentration trends are consistent.Then, the effect of FO membrane on the retention of antibiotics and antibiotic resistance in the effluent from the two settling tank of AAO system was explored. The effects of different concentrations of solute and solute concentration and flow rate on the antibiotic were investigated.Under the optimized conditions, the FO membrane was used to carry out the interception of antibiotics and antibiotic resistant bacteria in the water. Under the FO mode and PRO mode, the retention of SMZ and TC of the three kinds of FO membrane were MgSO4> C6H12O6> Na Cl under different solute solute conditions. When the draw solution of NaCl concentration from 0.5to 1 5 mol, CTA-ES membrane and TFC-ES membrane of TC and SMZ interception rate increased gradually in fo mode, the film CTA-NW on TC and SMZ interception increased firstly after slightly. In PRO mode, the retention rate of TC and SMZ in the three kinds of FO membrane showed a gradual increasing trend. When the flow rate is from 8 cm?s-1 to 24 cm?s-1, in FO mode,the rejection rate of TC and SMZ in three kinds of FO membrane increases with the increase of the flow rate in FO mode. In PRO mode, the CTA-ES film and TFC-ES film kept increasing trend,but the retention rate of CTA-NW and SMZ was increased first and then decreased. Under the optimum operating conditions, the maximum retention of SMZ and TC was 96.88%(CTA-ES,PRO) and 96.38%(CTA-ES, PRO) in three FO membrane. The interception rate of ARB in the two settling tanks of three FO membrane was more than 90%. Three kinds of FO membrane on tet(C) interception of the best, tet(B) and tet(M) retention is poor.On this basis, in order to further improve the anti fouling capacity and rejection rate of FO membrane, the anti fouling ability of dopamine modified FO membrane and its ability to remove antibiotics and drug resistance were investigated. Bovine serum albumin(BSA), TiO2 and TiO2 were mixed as raw material, and the water flux attenuation rate of the modified membrane was significantly lower than that of the original film. 4 kinds of membrane cleaning water flux recovery rate can reach 90%, the modified membrane has a stronger ability of membrane stability and anti pollution. In FO mode, the retention rates of TC and SMZ were 95.56% and 94.92% by DA-CTA-ES membrane. The rejection rates of TC and SMZ were 94.52% and 92.43% by DA-TFC-ES membrane, respectively. In PRO mode, the rejection rates of TC and SMZ were96.86% and 96.24% by DA-CTA-ES membrane. The retention rates of TC and SMZ were 95.92%and 94.43% by DA-TFC-ES membrane, respectively. The retention rates of SMZ and SMZ were and respectively. Compared with the original FO membrane, the retention rate of ARB has increased by dopamine modified FO membrane. |
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