Determination Of Some Kinds Of Organic Substances In River Water Using Method Of Chemiluminescence Coupled With Molecular Imprinted Technology

With the rapid development of industry and agriculture, the ability of environment to deal with the rubbish has reached its final limitation. And then, how to deal with the polluted water is the most important problem about environmental governance. For most of organic pollutants, which are mutagenic, carcinogenic and highly toxic poisons,it is also much more difficult to reduce and control after accumulation day and night. So that, the people’s life and the human’s surviving has been threatened by this pollution.Therefore, it is necessary and meaningful to build a novel analytical method of separation and determination of the pollutants in water online.Molecular Imprinting Technology(MIT) is a new kind of preparation method of polymer, which has the advantages of specific recognition ability, high selectivity, good stability in alkali or acid environment and long life-time. This technology has been widely applied on environmental detection, food industry, separation, chromatography and other fields. Chemiluminescence(CL) has became a very promising analytical technique for its advantage, such as high sensitivity, wide linear range, fast analytical speed, cheap and simple equipment and so on. But for complicated samples, CL will suffer from the interference problem of other similar substances, that will limit its application on complicated environment. Now, this poor selectivity was solved by combining MIT and CL. In order to reduce the interference from other substances, the molecular imprinting polymers were prepared and connected into the CL flow pass,which is mainly charge of enriching target molecular and reduce interference from other substances.This paper mainly has carried on research work, as follows:1. Using surface molecular imprinting technology, with silicon dioxide as a carrier,KH-570 as modifier, and hydroquinone(HQ) as imprinted molecular, a kind of HQ molecular imprinted polymers was synthesized, which is able to selectively recognize HQ. Based on the intense sensitization of HQ on CL system of KMnO4-PPA, the polymers were prepared molecular imprinting column, and connected into the flow of chemiluminescence as chemiluminescence instrument. And then, a novel method of determination of HQ in river water using MIT-CL technology was established and obtained successfully application.2. Using surface molecular imprinting technology, with silicon dioxide as a carrier,3-nitroaniline molecular imprinted polymers were synthesized, which was of good recognition to 3-nitroaniline and adsorption capacity of 42.8 μg/mg. The polymers were made into molecular imprinting column, and connected with the station of sampleinjection, to enrich the target molecular and elute impurities. And then, a novel method of determination of 3-nitroaniline in river water using MIT-CL technology was established and obtained successfully application.3. Based on the strong sensitization effect of chloroaniline of this chemiluminescence system of nickel(Ⅳ) coupled with luminol, a novel flow injection- chemiluminescence method for determination of residual chlorine aniline in the river water was established.In this paper, the synthetic method of surface molecular imprinting was using, and MIT and CL analysis were combined to deal with the problems about single chemiluminescence with poor selectivity and difficult application on complex samples.So, this MIT-CL technology have broad application prospects in waste water treatment with the advantages of both CL, which behaved high sensitivity, rapid analysis and low cost, and MIT, which production behaved good adsorption ability, acid and alkali resistant, and inexpensive, can be recycled many times.