The Application Of Two Kinds Of Chemiluminescence Functionalized Nanoprobe In Electrochemiluminescence Analysis

Electrogenerated chemiluminescence analysis has attracted more and more attention of scientists as a high sensitive analytical method. The scope of its application in biological analysis research was reviewed. Chemiluminescence functionalized nanomaterials has both of chemiluminescence characteristics and nanomaterials size effect, good bio-compatibility, large specific surface area and other properties. Therefore, its synthesis and analysis application has also become a research focus in recent years. Chemiluminescence functionalized nanomaterials not only has characteristics of luminescence and nanometer materials, and easy to immobilized biological probe in the surface area such as DNA, protein, etc. In this study, two kinds of electrochemiluminescence functionalized nanomaterials were synthesized and applied to the analysis and determination of dopamine and kanamycin. The main research content is as follows:1. Luminol functionalized gold nanoparticles were synthesized by using the methods of literature has been reported. Electrochemiluminescence was applied to the determination of dopamine based on luminol functionalized gold nanoparticles modified electrode. An electrochemiluminescence sensor was prepared based on luminol functionalized gold nanoparticles. The electrochemiluminescence behavior of luminol functionalized gold nanoparticles modified electrode was investigated, and it was found that the modified electrode exhibited excellent ECL performance. The modified electrode was used as sensor in 0.02mol·L-1 Na2CO3-NaHCO3buffer solution (pH=10) with 1.0×10-3 mol·L-1 H2O2 solution. Under the optimal experimental conditions, the ECL intensity of the sensor exhibits a wide dynamic range from 1.0×10-10～1.0×10-5mol·L-1 with a low detection limit of 6.3×10-11mol·L-1 for the determination of dopamine. The proposed method was applied to the analysis of dopamine in urine sample, giving values of recovery in the range of 102%-104%.2. Go-ABEI-hemin-Au functionalized graphene hybrids were synthesized by using the methods of literature have been reported.An electrochemiluminescence sensor was prepared based on Go-ABEI-hemin-Au functionalized graphene hybrids bind with kanamycin aptamper of the detection for kanamycin residue in milk samples.The electrochemiluminescence behavior of Go-ABEI-hemin-Au functionalized graphene hybrids modified electrode was investigated, and it was found that the modified electrode exhibited excellent ECL performance.It exhibits a wide dynamic range from 1.0×10-11 mol·L-1～1.0×10-6mol·L-1 with a low detection limit of 5.7×10-12 mol·L-1.The proposed method was applied to the analysis of kanamycin in milk sample, giving values of recovery in the range of 95.2%-103.6%. The proposed aptasensor has some advantages such as simple operation, high sensitivity, no need of complex labeling and purification steps, making this method faster and easy automation.