Immunoprotection Of S. Aureus IsdB, TRAP, And ClfA Fusion Protein

Staphylococcus aureus is an important pathgen of human and animals. However, treatment for infection of S. aureus is more difficult during endurance strain continuously emerging. Vaccines become more important method for prevention and control that. Recently, some S. aureus structure proteins and virulence factors, such as IsdB, IsdA, Trap, Rap, ClfA, FnbP, and Can, were used as vaccine antigen candidates in researches. Immune response of host could be induced by these antigens, but immunoprotection effection still far away from ideal. In order to improve candidate antigens’immunoprotection effection, S. aureus IsdB, TRAP, and ClfA, which were found good immunogenicity, were fusion expressed to study production’s immune response and immunoprotection role for providing a new candidate antigen for vaccine research.ClfA_N2N3 was clipped into two fragments, ClfA_N2 and ClfA_N3, through prokaryotic expression, emulsification with Freund’s adjuvant, and immunization in mice to check their immunoprotection against S. aureus challenging as immunogens. Either whole fragment, ClfA₍N2N30, or single clipped fragment, ClfA_N2 or ClfA_N3, could induce immune response after immuning in mice. After challenging by S. aureus Newman or HLJ23-1, survival percentage of group mice immuned with ClfA_N3 or ClfA_N2N3 was similar. Then, ClfA_N3, IsdB_id, and TRAP were fused expression. The fusion production was named ITC, and ITC was emulsificed with Freund’s adjuvant for immuning mice to check its immunoprotection role. As results, ITC could induce mice’s immune response. After challenging by Newman, HLJ23-1, or Wood46, survival percentage of mice in ITC immune group was higher than other groups. Recombination ITC protein could play better immunoprotection function, and could be used as a candidate for further defeating S. aureus infection vaccine.