| Objective:By using homogenous design method for alisma orientalis tiaozhi particles disassemble square research, explore alisma orientalis tiaozhi particles tiaozhi dose best compatibility proportion, expounds the mechanism of action of treatment hyperlipidemia, for compound optimization research, development new drugs to lay the foundation.Methods:1. The theory research:from traditional Chinese medicine and modern medicine two aspects, this paper expounds the tall lipemia and treatment of the researches. Adopt documents and analysis method of traditional Chinese medicine, detail hyperlipidemia etiology and pathogenesis, clinical classification, syndrome differentiation and treatment of understanding, retrospected the modern medical hyperlipidemia research progress. Combining the traditional theory and modern pharmacology study results of alisma orientalis modulation particle party card relations and fang disease relationship is analyzed theoretically.2. Experimental research:using high fat emulsions gastric lavage copy hyperlipidemia rat model, according to the uniform design requirement of experiment, different dose group dosing filling stomachs after 2 weeks, abdominal aortic blood lipid, combined with the four fundamental index, through computer software are analyzed and regression equation and the best ratio, and validate experiments, again detection weight, liver index, the four cardinal lipid learn index and liver tissue morphological changes, confirmed the optimum ratio of computer analysis is correct.Results:1. The theory research shows that tall lipemia main pathogenesis for diet partial eosinophilic, taste damage; Life smooth, yixian little work, Sentiment not free, internal organs disorders, Phlegmy turbidity yu, angry machine obstruction. This disease, kidney, liver and spleen three dirty close relationship, especially in the spleen is a center. According to the pathogenesis of this disease characteristics, the tutor combined with years of clinical experience and the TCM literature material, put forward the disease incidence of pixu bowls, key lies in the wet phlegm and blood block blood. The pathogenesis of this disease is phlegmy wet key, is also the disease development and change of the center link, puts forward enriching qi, eliminating phlegm to remove stasis the treatment. And by "golden cabinet of alisma orientalis soup basis, combining the characteristics of Chinese herbal medicine, fujian and Taiwan researchason alisma orientalis modulation particles, the treatment of hyperlipemia card to belong to phlegm and blood stasis, spleen qi stag. type patients with reluctance.2. Experiments have calculated by the regression equation knowable, TC and TG with the size of the factors A and B, factors C factors, and are in close relation linear correlation, explain index size, mainly depends on each factor for A given range value, and as A monarch, minister ErYao factors A and B factors in lowering blood fat, throughout negatively correlated with the indexs, have main effect, show that the party is accord with TCM recipe theory of compatibility and Chinese medicine pharmacology modern research objective laws. Alisma orientalis tiaozhi grains in the favorable king-subject medicine than 2.5:1, for reducing hyperlipemia rats TC effect is best, when the ratio of the favorable king-subject adjuvant for 17.5:now, for reducing hyperlipemia rats TG best effect.Conclusion:1. The theory research shows that alisma orientalis tiaozhi particles reagent accord with "party card consistent" the compatibility of the concept and "disease and syndrome coherent" research ideas, have enriching qi, eliminating phlegm to remove stasis efficacy, and can be used for tall lipemia enhanced of phlegm and blood stasis block, spleen lost health shipped patients.2. Pharmacodynamics experiment showed that, in the uniform design method, alisma orientalis tiaozhi grains in the favorable king-subject medicine than 2.5:1, for reducing hyperlipemia rats TC effect is best, when the ratio of the favorable king-subject adjuvant for 17.5:now, for reducing hyperlipemia rats TG best effect. With the normal group and model group compared, alisma orientalis tiaozhi particles best TC ratio group, best TG ratio group, can effectively reduce the TC and TG, LDL-C level, and improve hdl-c leel, a significant difference (P<0.01). Hepatic pathology observation showed optimal proportion of group can reduce tall lipemia rat liver pathological damage, reduce steatosis liver cell number and pathological damage. Can confirm optimal proportion of group can replace the square, in its lower TC and TG etc., effective group is divided into alisma orientalis, white atractylodes etc many kinds of modern Chinese medicine pharmacology has been proved to be lipid-lowering function of Chinese traditional medicine. |
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