Analysis Of Carbamates And Plant Hormones In Foods And Water By High Performance Liquid Chromatography

Along with the development of the economy and the society, and the improvement of thepeople’s living level, people pay more and more attention on food quality. Due to the foodsafety problem occurs frequently in recent years, more efforts should be made towards thefood quality detection. In the first chapter of the paper, we summarized the characteristics andhazards of the carbamate pesticides and plant growth regulator, and introduced the mainanalysis methods to these two kinds of pollutant. In the second chapter, the methods for thedetection of the carbamate pesticides were established. A simple and efficient liquid phasemicroextraction method-floated organic drop microextraction (FDME) coupled with highperformance liquid chromatography for the analysis of carbamate insecticides in water wasdeveloped. The components were separated by a Diamonsil C18analytical column (250mm×4.6mm i.d.,5μm). The mobile phase was a mixture of acetonitrile, methanol, and water (theratio of volume was17:40:43). The limit of detection (LOD) for this method was1-4ng/mL.The impact of extractant, extraction time, volume of the single organic drop, stirring speedand other conditions on analysis result were investigated. In the third chapter，the solid-phasedispersive extraction with high performance liquid chromatography was applied for theseparation and determination of α-naphthylacetic acid and p-chlorophenoxyacetic acid inketchup. The anhydrous sodium sulfate was the dispersing agent in the process of solid-phasedispersive extraction. The components were separated by C18column in the wavelength at230nm. The mobile phase was methanol and water (50:50). The calibration curve was linearbetween0.005and5.0mg/mL, the correlation coefficient were greater than0.999, recoverieswere87.0%and71.0%, respectively. In the last chapter, the method which was used dynamicsolid phase microextraction coupled with HPLC for the detection of three estrogens in milkwas discussed.The adsorbents were put into the package which was builded usingpolypropylene. The components were separated by a Diamonsil C18analytical column(250mm×4.6mm,5μm) in the wavelength at230nm. The mobile phase was a mixture ofacetonitrile, methanol, and water (the ratio of volume was40:20:40). The pH was adjustedby methanoicacid until it was five. The calibration curve were linear between0.5and100μg/mL, the correlation coefficient were0.9996.