Expression And Clinical Significance Of PTEN In Normal Brain Tissue And Gliomas

Objective：To detect the PTEN (also known as MMAC1and TEP1) of the normal brainand glioma in the gene and protein level，explore the relationship between PTEN and thelevels of, glioma, providing theoretical and experimental evidence for glioma’s diagnosis andtreatment.Methods: Collecting of38pathologic specimens of glioma operation treatment of theAffiliated Hospital of Hebei University Neurosurgery in February,2010~January,2012andcollecting15cases of normal brain specimens from intracranial hypertension afterdecompression operation. All glioma and normal brain tissue specimens untreated salinesoaks, was collected immediately after the liquid nitrogen10min, to the refrigerator for-80degrees C. Tumor tissue by WHO glioma grading standard content grade I8cases,24cases ingrade II,29cases in grade III and grade IV7cases, in which the grade I and the grade IIreturn for low level group, grade III^IV as high level group. All tumors were confirmed thediagnosis by pathology laboratory. This experiment by using reverse transcription PCR(RT-PCR), immunoblotting(western blot) and immunohistochemistry (S-P) methods wereused to detect the expression of PTEN in human brain glioma and normal brain tissueexpression.Results: immunohistochemical method: The positive rate of normal brain tissueexpression is100%；The high level group was16cases, the positive rate of35.29%; low levelgroup was6cases, the positive expression rate of76.19%; immunoblotting method: proteinexpression of poorly differentiated group was0.579±0.192, protein expression of highdifferentiation group amount of0.197±0.069; RT-PCR method: mRNA expression of poorlydifferentiated group amount of2.997±0.593and high differentiation group is1.237±0.253.The experiment onfirmed that the expression of PTEN in mRNA, protein and tissuelevels is matching. The expression in normal brain tissue is higher than in glioma and it isnegatively correlated with human glioma level. The difference is statistically significant.(P<0.01). With the glioma differentiation degree increasing, the expression of PTEN graduallyrises. Conclusion: This experiment using three kinds of test methods in gene,protein andtissue level in human brain glioma and normal brain tissue detect the expression of PTEN andanalysis of the correlation between PTEN and glioma grade. Study found that, in the normalhuman brain PTEN expression rate is100%and with the different levels of glioma, PTENexpression positive rate is varies. With glioma pathological grades increased, the positive rateof PTEN expression decreased, suggesting that the positive rate of PTEN expression isnegative correlation with the pathological grades of glioma. With pathology of the higherlever, the glioma patients’ expression of PTEN is less. Pten may be potentially importantbiological indicators affecting the occurrence, development and prognostic of glioma patientsand is expected to become the new direction of glioma patients.