Effects Of Postconditioning With Propofol And Remifentanil On Hepatic Ischemia Reperfusion Injury In Rats

Objective To investigate effects of postconditioning with propofol and remifentanil onhepatic ischemia reperfusion injury (HIRI) in rats.Methods Fifty male SD rats weighing220-280g were based on the random digits table,divided into5groups of10animals each randomly: sham operation group (groupⅠ), I/Rgroup (groupⅡ), propofol postconditioning group (groupⅢ); remifentanil postconditioninggroup (group Ⅳ), propofol combined with remifentanil group (group Ⅴ). In group Ⅱ~Ⅴ thehepatic arteries and veins of middle and left lobes were occluded for30min, and group Ⅰwasan open surgery only. In group Ⅲ Ⅳ and Ⅴ porpofol (at a rate20mg·kg-1·h-1for1h)、remifentanil (at a rate1μg·kg-1·min-1for1h) and porpofol (at a rate20mg·kg-1·h-1) combinedwith remifentanil (at a rate1μg·kg-1·min-1for1h) was infused iv at the onset of reperfusion.In groupⅠ and Ⅱ normal saline was infused iv at the onset of reperfusion at a same rate.Blood samples were taken at the end of1h reperfusion for determination of serum AST andALT activity, liver tissue immunohistochemistry, scanning electron microscopy observe liverhistopathological injury.Results Compared with GroupⅠ, the activity of AST and ALT of Group Ⅱ~Ⅴ wereall increased, the gene of c-fos and c-jun expression-positive cell rate of liver were higher (P<0.05or0.01); Compared with Group Ⅱ, the activity of AST and ALT of Group Ⅲ~Ⅴ werelower, the gene of c-fos and c-jun expression-positive cell rate of liver cut (P <0.05or0.01);Compared with Group Ⅲ, GroupⅣ and Ⅴ in serum AST and ALT, the gene of c-fos andc-jun expression-positive cells has no difference (P>0.05); Compared with Group Ⅳ,GroupⅤ in serum AST and ALT, the gene of c-fos and c-jun expression-positive cells has no difference (P>0.05); Scanning electron microscopy showed that Group Ⅲ~Ⅴpathologicalinjury of liver tissue was significantly reduced compared with Ⅱ.Conclusion Propofol or remifentanil can attenuate HIRI and no difference between thetreatment with propofol or remifentanil and the treatment of propofol combined withremifentanil, the mechanism was relevant to inhibiting apoptosis in rat liver.