The Significance Of Chromosomal Analysis Among Patients Undergoing IVF/ICSI

Objective To study the association of chromosome abnormity withreproductive abnormalitie and IVF/ICSI outcomes; to provides basis for theheredity consultation and clinica treatment decision.Method The chromosome karyotype were analysed for638infertile couplesundergoing IVF/ICSI retrospectively. The prevalence of chromosomalabnormalities among these patients was culculated. Patients were divided into twogroups: the normal and the abnormal chromosomal karyotype groups, karyotypeswere grouped by their abnormal types and gender. The IVF/ICSI outcomes of thenormal and abnormal karyotype groups were compared; The association of semenquality and quantity were analysed with different Y chromosome abnormality;The corelation of MII oocyte with female abnormal karyotypes were anayzed.Results:①Out of638infertile couples who underwent IVF/ICSI in our study,518with normal karyotype,128with abnormal chromosome, The prevalence ofchromosomal abnormalities is10.3%, the abnormal karyotype are more commonin male patients(101cases) than female’s(27cases), the proportion is3.74(101/27)between male and female patients.②We found that the small Y chromosome wassignifcant higher in mild to moderate oligo-asthenospermic patients than that innormal semen patients (6.1%vs1.6%)(P<0.05); the small Y chromosome was alsohigher in azoospermia patients than that in normal semen group (6.1%vs1.6%)(P <0.05). The big Y chromosome was higher in severe or very severeoligo-asthenospermic patients than that in normal semen patients (17%vs 6.4%)(P <0.05);③Compared with the normal chromosome types group, thefertilization rate58.6%, the cleavage rate90.6%, the high quality embryo rate47.2%in abnormal karyotype group were lower than thats in normalchromosome types group70.5%,99.2%,60.7%(P <0.05); The cleavage rate96.4%and the birth rate18.2%in big Y chromosome group were lower than thats innormal chromosome types group99.2%,39.1%(P <0.05), the abortion rate57.1%was higher than thats in normal group26.3%(P <0.05); the cleavage rate96.3%and the high quality embryo rate52.8%in small Y chromosome group werelower than thats in normal group99.2%,60.7%(P <0.05); the high quality embryorate in Inv (9)/(1) group was lower than that’s in normal group(48.4%vs60.7%)(P<0.05); the cleavage rate93.8%, the high quality embryo rate47.6%in the1,9,16chromosome heteromorphisms group were lower than thats in normal group99.2%,60.7%(P <0.05); the fertilization rate60.3%, the cleavage rate94.3%inthe D/G group were lower than that of the normal group70.5%,99.2%(P <0.05).④In Inv (9)/(1) group, the high quality embryo rate of the female groups were58.1%, it was higher than thats in normal groups60.7%(P <0.05); in the1,9,16chromosome heteromorphisms group, the fertilization rate of the female group was60.3%, it was lower than the male group74%(P<0.05). In the D/G group, thefertilization rate of the female group was56.5%, the cleavage rate of the femalegroup was92.3%, both of them were lower than that of the normal group70.5%,99.2%(P <0.05).Conclusion:①The prevalence of chromosome abnormality in patientsundergoing IVF/ICSI is significantly higher than reported rate among normalpopulation.②The big or small Y chromosome karyotype is related toasthenospermia, azoospermia.③The types and gender of abnormal chromosomekaryotype affected IVF/ICSI outcomes.