Effect Of The DFC On Intestinal Mucosal Barrier In Mice With SAP

Research background:Severe acute pancreatitis is a disease which has the features of acute onset, severe illness status, complications and high mortality. It relates systemic response syndrome, infections and bacteremia. Therefore, There is a clear evidence that this secondary bacterial infections is due to intestinal bacterial replacement caused by function of intestinal mucosa damage when SAP. How to prevent and reverse intestinal mucosa barrier damage has the important meaning to SAP effective treatment. Dietary fiber (DF) is the sum of a few lignin and polysaccharides which come from plants and can’t be hydrolyzed by digestive enzymes in the small intestine. It mainly includes the cellulose, hemicellulose and material of pectin. DF can be divided into two classes according to water-soluble:one kind is soluble dietary fiber, another kind is insoluble dietary fiber. They have stimulative effect to the small intestine, colonic mucosa growth and cell proliferation. Dietary fiber complex (DFC) can maintain the normal form of bowel mucosa, protect bowel mucosa barrier, prevent bacteria translocation. So, this subject is to evaluate Effect of the DFC on intestinal mucosal barrier in mice with SAP, so that it can provide a basis for clinical application.Objectives:To explore the effect of the DFC on expression of intestinal mucosal on tight junction protein and MLCK in mice with SAP, further research the influence of Dietary fiber complex (DFC) on intestinal mucosal barrier with SAP, understand of the dietary fiber complex’s protection on intestinal mucosa, provide theory support for clinical application of enteral nutrition.Methods:1. SAP mouse model was established with5%sodium taurocholate which was injected into pancreaticocholang retrogradely, the silicon tube whose outer diameter is1.5mm was placed into the jejunum through the pylorus area, fixed to the stomach wall, derived from subcutaneous tunnehe of neck, fixed to the neck skin.SAP mice were randomly assigned to3groups: SAP+enteral nutritional emulsion (fresubin) group(A group),SAP+fresubin and DFC group (soluble dietary fiber SDF:insoluble dietary fiber IDF=2:1, B group), SAP+fresubin and DFC group (SDF:IDF=2:1, C group). Different enteral nutrition which was given by four times through the injector according to125ml/(kg. d) was given to the mice six hours After surgery. Each group contained20mice, they freely drank physiological saline during breeding period. 2. The mice were executed on48h after surgery.Pathological intestinal mucosal changes, tight junction protein occludin、ZO-1and myosin light chain kinase(MLCK) were evaluated in all groups. The intestinal mucosal pathological changes were dyed by HE, The rest of the index were evaluate through DAB color development after Streptavidin and Peroxidase.3. The statistical analysis was done through SPSS16.0statistics software, measurement data were indicated with the mean±standard deviation, the comparison between the group were analysed by variance analysis, the two-two comparison were with the q inspection,Count data were with the fisher exact test.Results:1. Intestinal mucosal injury:Intestinal mucosal injury in B and C groups was lighter than that in group A(P<0.05).Intestinal mucosal injury in group C was lighter than that in group B (P<0.05).2. Tight junction protein:Tight junction protein occludin and ZO-1in B and C groups express higher when compared with group A(P<0.05),furthermore, occluding and ZO-1in C group express higher than those in group B(P<0.05).3. Myosin light chain kinase (MLCK):MLCK in B and C groups express higher than that in group A(P<0.05), MLCK in C group express higher than that in group B, but the difference is not statistically significant (P>0.05)Conclusion1. Enteral nutrition (EN) containing dietary fiber complex (DFC) can effectively reduce the intestinal mucosal damage of the pathology, improve the intestinal mucosa tight junction protein and MLCK’s distribution and expression, can effectively protect the intestinal mucosa.2. Different proportions of the SDF and IDF has different protection on intestinal mucosa, the enteral nutrition which moderate increase the proportion of IDF is better than that which has lower proportion IDF, much effectively protect intestinal mucosal barrier.