The Cell Classification And The Level Of TNF-α, IL-6, IL-10in Bronchoalveolar Lavage Fluid Of Rats Model With Chronic Obstructive Pulmonary Disease In Different Altitudes

Objective: To study the cell classification and the level of tumor necrosis factoralpha (TNF-α), interleukin6（IL-6）,interleukin-10（IL-10）in broncho-alveolar lavage fluid of rats model with chronic obstructive pulmonary disease(COPD) in different altitudes. In order to know the influence of hypoxia onbronchial inflammation of COPD.Methods：Constructed the model of COPD with the method of intraperitoneal injec-tion of cigarette smoke extracts. Sprague-Dawley(SD) rats (n=50) were randomlydivided into5groups：control group, saline group and COPD group in moderatealtitude, saline group and COPD group in high altitude.Observing the cell classificationwith the method of Pap stain and the level of TNF-α, IL–6, IL–10with the method ofenzyme linked immunosorbent assay (ELISA) in bronchoalveolar lavage fluid of each group.Results： The proportion of macrophage in COPD group in moderate and highaltitude were higher than saline group in same altitude（P<0.01）. The level of TNF-α,IL-6in saline group in high altitude was higher than saline group in moderate altitude（P<0.05）;The level of TNF-α,IL-6in COPD group in moderate and high altitudewere higher than saline group in same altitude（P<0.05）.There was no significantdifference of the level of IL-10among5groups.Conclusions：1.Hypoxia increased the proportion of macrophage,the level of TNF-αand IL-6in bronchoalveolar lavage fluid in health rats in high altitude.2.Hypoxia didn’t influence the bronchial inflammation in COPD rats in high altitude.3.The role of IL-10in bronchial flammation of COPD and hypoxia in high altitudeneed to do further research..