The Study On Clinical Significance Of Expression And Relation About MMP-2and TIMP-2in Patients With Gastric Carcinoma

Objective To detecting the expression of MMP-2and TIMP-2in gastric cancer,study MMP-2and TIMP-2expression in gastric cancer clinical relationship, to furtherexplore the invasion and metastasis of gastric cancer.Methods Collected from Qinghai Province People’s Hospital of Department ofGeneral Surgery and Surgical Oncology implementation of surgical resection and thepostoperative biopsy confirmed50cases of gastric cancer specimens in May,2010–October,2012. Using2010International Union Against Cancer/American Cancer JointCommittee (UICC/AJCC) TNM staging criteria: Ⅰof10cases, Ⅱ of16cases, IIIof18cases, IV of6cases; depth of invasion: the serosa no infiltration21cases, serousinfiltration in29cases; histological types:16cases of well-differentiated, moderatelydifferentiated12cases, poorly differentiated in22cases; lymph node metastasis in23cases,27cases without lymph node metastasis. Using SABC immunohistochemicaltechniques (immunohistochemistry) to detect MMP-2, TIMP-2expression in50patients with gastric cancer in gastric carcinoma and peritumoral non-neoplastic gastricmucosa in the case.Statistical Methods: Using SPSS17.0statistical software to analyze the data. Countdata using the X2test and correlation analysis. Significance level alpha=0.05.Ruselts Using2010International Union Against Cancer/American Cancer JointCommittee (UICC/AJCC) TNM staging criteria: Ⅰof10cases, Ⅱ of16cases, IIIof18cases, IV of6cases; depth of invasion: the serosa no infiltration21cases, serousinfiltration in29cases; histological types:16cases of well-differentiated, moderatelydifferentiated12cases, poorly differentiated in22cases; lymph node metastasis in23 cases,27cases without lymph node metastasis.①MMP-2expression in gastric cancertissue is70.0%positive, while in normal one is10.0%positive with X~2=39.72andP<0.05which caused so significant differences. MMP-2positive rate in serosalinfiltration group is93.1%which is higher than that in the serosal non-filtration group(43.0%) with X~2=15.253and P<0.01and the difference is so obvious aftercomparision. MMP-2has a higher positive rate (91.3%) in lymph node metastasisgroup than in no lymph node metastasis group which is52.0%with X~2=9.206andP<0.05showing significant differences. MMP-2positive rate is96.0%in stageⅢ+Ⅳgroup, higher than in stageⅠ+Ⅱgroup (61.5%) with X~2=8.554and P<0.01showingsignificant differences.②TIMP-2expression in gastric cancer group is30.0%positive,and in normal one is34.0%positive showing no obvious differences. TIMP-2positiverate in the serosal non-filtration group is85.7%, higher than51.7%in serosalinfiltration group with X~2=6.27and P<0.05, showing significant differences. TIMP-2has a higher positive rate (74.1%) in no lymph node metastasis group than65.2%inlymph node metastasis group with X~2=9.628and P<0.05. Their differences are soobvious after comparison. TIMP-2positive rate is80.0%in stageⅠ+Ⅱgroup, higherthan46.0%in stageⅢ+Ⅳgroup with X~2=5.122and P<0.05showing significantdifferences.③MMP-2and TIMP-2in gastric carcinoma has a correlation of MMP-2expression was positively correlated with the expression of TIMP-2, confirmed thatTIMP-2expression in gastric cancer increase with increased expression of MMP-2expressionConclusions①MMP-2that might be related with the progress of gastric cancer.②Inhospital,By detecting the expression levels of MMP-2in gastric cancer patients canpredict the relationship of invasion、 metastasis and prognosis of gastric cancer.③MMP-2/TIMP-2ratio as an indicator of tumor malignancy and prognosis.