Effect Of Dachengqi Decoction On Inhibiting The Gastrointestinal Motility Disorder Induced By Inflammation

Gastrointestinal motility disorder is the most important complications of peritonealinflammation, such as paralytic ileus and its induced gastrointestinal necrosis. Inhibitionof the gastrointestinal motility disorders induced by peritoneal inflammation is animportant step for prevention and treatment of related complications, but the drugs appliedin clinic have little effect. It is urgent for discovering drugs with better effects and fewerside effects.Traditional Chinese Medicine, with good curative effect, rich source and less sideeffects, gains more and more attention in the treatment of gastrointestinal motility disorderin recent years, but the unclear underlying mechanisms limits its useness. Dachengqidecoctions of Zhang zhongjing is a classic prescription with the effect of purging thebowels and removing heat. In modern medicine aspect, Dachengqi decoction can promotegastrointestinal motility disorder and inhibite inflammation. Therefore, we wonderwhether Dachengqi decoction could be ideal medicine in the treatment of gastrointestinalmotility disorder diseases. It is suggest that interstitial cells of Cajal (ICCs) are the origin of gastrointestinal rhythm in the gastrointestinal tract by generating the slow waveof gastrointestinal smooth muscle. Our previous studies have found that distribution ofHCN in ICCs and HCN lead autorhythmicity rhythm of ICCs. But there is no researchabout effect of Dachengqi decoction on regulating HCN channel.Our previous studieshave confirmed that extract of Dachengqi decoction had effect on promotinggastrointestinal motility and inhibiting inflammatory reaction, but its mechanisms stillneed further clarification. In this study, we used the intraperitoneal injection of LPS toestablish animal model of peritoneal inflammation. Then, the effects of Dachengqidecoction on activation of HCN1and influence of the inflammation were detected byimmunofluorescence histochemical, electrophysiology and molecular biology assay toexplore the mechanisms of Dachengqi decoction on promoting gastrointestinal motility.The results may provide the theory basis for clinical development of Traditional ChineseMedicine.Methods:(1) Establishing rat model of peritoneal inflammation induced by LPS, A:normal control group; B: model group; C: drug intervention (Dachengqi decoction) group;the changes of gastrointestinal motility were detected by electrophysiological method toobserve;(2) Using electrophysiology assay to observe the changes of model ratsgastrointestinal motility at different times after intervention of Dachengqi decoction;(3)Detection the changes of HCN1expression by immunohistochemical staining;(4)Studying the effect of Dachengqi decoction on expression of NF-κB and inflammatoryfactor levels;(5) Analyzing the correlation between inflammatory factor levels andgastrointestinal motility.Results:(1) With the progress of inflammation of model rats (preliminary stage,1~3d), thegastrointestinal motility wore off; the activity of HCN1weakened gradually, and levels ofinflammatory factor increased. Compared with the control group, the difference issignificant. After treated by Dachengqi decoction, the results showed significant differencecompared with whether the experimental group or the control group also exist differences;(2) In the middle stage (5~7d), especially in the3d, the gastrointestinal motility weakenedsignificantly and activity of HCN1is most obvious. The inflammatory factor and NF-Κ B levels decreased significantly in the5d, reduce the level of the most significant. In the7d, compared with normal control group, all indexes above are not statistical significance.And there was a negative correlation between pro-inflammatory factor levels andgastrointestinal motility, while a positive correlation between anti-inflammatory factorlevels and gastrointestinal motility.Conclusion: Dachengqi decoction has a significant effect on promoting gastrointestinalmotility by activating the HCN1of ICC. It also has anti-inflammatory effect throughregulating NF-κB which is an important molecule in cell signal transduction pathways ofinflammation.