Study On Preparative Isolation And Purification Of Active Ingredients From Traditional Herb Scutellariae Barbatae And So On

The rapid development of technology has promoted the progress of medical level; itmeans that the requirements of the traditional Chinese medicine become increasinglyhigher. The traditional Chinese medicine contains many chemical components undernormal conditions but the ingredient of the traditional Chinese medicine is single, so it’sextremely important to establish an efficient method to purify and separate the ingredientsof traditional herb. In this paper, in order to find an efficient and simple method to purifyand separate the ingredients of traditional herb we optimized the conditions of thisexperiment.1. Study on preparative isolation and purification of flavonoid compounds fromtraditional herb Scutellariae Barbatae.A method to separate and purify the flavonoid compounds from Scutellariae Barbataeby polyamide column chromatography and PHPLC was established. The conditions ofextraction process including extraction methods, extraction solvent, extraction times, andthe concentration of extraction solvent were optimized. The crude extract dissolved withwater was chromatographed on polyamide resin in order to pre-separate the ingredients ofScutellariae Barbatae. The PHPLC was used to further purify the components. Conditionsof PHPLC: column: GLP-C18column（400mm×25.4mm ID20um）mobile phase:methanol-water; detection wavelength:290nm. Under this simple and convenient methodsix flavonoid compounds have been separated from crude extract. Those purified flavonoidcompounds were apigenin-5-O-β-D-glucopyranoside、 naringenin、suctellarein、luteolin、5,7,4’-trihydroxy-8-methoxyflvone、apigenin with purities of96.8%、95.6%、96.9%、99.4%、97.2%、98.4%. The chemical structures were confirmed by1H-NMR and13C-NMR.2. Study on preparative isolation and purification of active ingredients from RhizomaBelamcandae.A method to separate and purify the active components from Rhizoma Belamcandae by silicagel column chromatography and PHPLC was established. In this paper, silicagelcolumn chromatography has been used to separate the active components. The separatedproducts were further purified by PHPLC with methanol-water in different proportions.The conditions of PHPLC have been determined in this paper. The column was GLP-C18column（400mm×25.4mm ID20um）. Through this method eight kinds of compoundshave been obtained. They were isflorentin, irignin, Irigenin, apocynin,3’,5’-dimethoxyphenyl tectorigenin-4’-O-β-glucoside, resveratrol, tectoridin and iridin withthe purity of95.9%、97.2%、92.1%、93.8%、98.9%、95.0%、93.4%、99.0%. The chemicalstructures were confirmed by1H-NMR and13C-NMR.3. Separation and purification of soy isoflavonoids from soybean extracts by usingSOURCE15RPC as the stationary phase in PHPLC.A preparative high performance liquid chromatography method for separation andpurification of soy isoflavonoids from soybean extracts was successfully established byusing SOURCE15RPC as the stationary phase. Methanol-0.1%phosphoric acid ingradient elution mode was employed for separation. Five kinds of soy isoflavone includingdaidzin, glycitin, genistin, daidzein, genistein were obtained from soybean extracts. Thepurity of these compounds were98.6%,97.8%,99.3%,99.5%,99.1%, respectively. Theirchemical structures were confirmed by1H-NMR and13C-NMR.