| Objective：Observe the continuous gastrointestinal cleaning in paraquat(PQ)poisoning rabbits in the treatment effect.Methods：Select male Japanese white rabbit24, were randomly divided intoparaquat poisoning group (group A), pure gastric lavage group (group B), gastriclavage with A drug therapy group (group C) and gastric lavage with multiple drugtherapy group (group D). Set up the paraquat poisoning rabbits model, the rabbits inevery group were treated with paraquat which was dilution to10mg/ml at60mg/kg byintra-gastric. After two hours, the rabbits of group B C and D were treated by gastriclavage with the same volume of balance saline. Completed the gastric lavage, thegroup C was given3g smectite powder,125ml mannitol and0.3g activated charcoalin the first day. The rabbits of group D were given the same treatment in four days. Atthe same time of everyday, all rabbits were draw blood in the ear marginal vein. Theblood was used to the paraquat concentration and malondialdehyde(MDA). Allrabbits were executed by air embolism method and the lung tissues were obtained forpathological examination.Result：(1)General situation of the rabbits: the rabbits of group A had poor spiritand appetite, the group B had the same result; compared with the group A and B, theresult of group C and D was the opposite, and the group D had the best situation.(2)Changes of MDA content and PQ concentration in serum everyday: In the first day,the changes of MDA content and the concentration of PQ had a small difference; inthe4th day, Group A B C and D’s MDA content and concentration of PQ wasdescreasing, compared with group A and B, the group C and D was lower, and thegroup D’s was the lowest,(P﹥0.01).(3)Changes of MDA content and PQconcentration in serum at different days: The consequence about MDA content andPQ concentration in serum of group A and B was a little difference among the1th,2th,3th and4th; The first day’s consequence of group C was higher than other days,Group D’s concentration of PQ in serum decreased at each time points.(4)Changes ofhistopathological changes of lung tissue: To observe the lung of the group A and group B, there were appear dark red, the basis pulmonis can be observed the infarct.There were alveolar epithelial hyperplasia, alveolar lumen narrowed, proliferation offibrous tissue, pulmonary consolidation; The lungs of the group C were deep dark red,edema. Light microscope(HE) can find the lungs were multifocal bleeding,inflammatory cell infiltration; The lungs of the group D,which had normal alveolarframework, and the bronchial mucosa covering ciliated columnar epithelium. Thesewere bronchial mucosal chronic inflammation change. Pathological changes of thegroup D’s lungs is lighter than the group C’s.Conclusion：Gastric lavage is the first way of cleaning the gastrointestinal tractin poison. The effect of gastric lavage is limited, smectite and activated charcoal havea strong adsorption, the role of mannitol has a catharsis. Above these, they can cleanthe gastrointestinal tract. Gastric lavage combined with a drug treatment can removequickly PQ in the gastrointestinal at early therapy, and reduce the concentration ofparaquat. Compared with multiply drug treatment, it has a strong clearance ability,which can remove the residual concentration of PQ, delay the process of pulmonaryfibrosis. The multiply drug treatment can be the therapy method of paraquatpoisoning. |
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