Study Of Rapid Detection Of Glucose Based On Paper-based Microfluidic

Glucose is the indispensable material in human’s activities, its content is a measurement to the ability of metabolism and an index to monitoring the state of illness, especially in the field of clinical diabetes research is particularly important. Hence, establishing a rapid, economical, accurate glucose detection method has important significance to the human’s health, the disease’s prevention and diagnosis, also the treatment’s monitoring. Paper-based microfluidic chip technology possess lots of merits, such as it is easy to process, low cost, fast analysis, miniaturization, and portable, etc. Paper-based microfluidic chip technology and colorimetric analysis technology were combined effectively in this paper, a new method for rapidly detecting of glucose was set up and then used successfully in the detection of clinical blood sugar in practical samples.This paper was divided into three parts:Part one: Paper-based microfluidic chip’s starch-KI-gelatin system for glucose detectionThis section was based on a rapid and sensitive starch-iodine color technology, combined with the characteristics of paper-based microfluidic chip and catalytic reaction to glucose specificity by glucose oxidase, the properties of gelatin that was it would amplify signals in the solution and could be used as a protectant on the paper, through the scanner imaging and Adobe Photoshop software data processing the color results, established a new method which based on a starch-potassium iodide-gelatin system to measure H2O2and glucose, and used for quantitative analysis of glucose in serum. Results showed that after adding gelatin will make the starch-potassium iodide-hydrogen peroxide’s signal be greatly enhanced. Under the optimized experimental conditions, it was a good linear relation between absorbtion value and concentrate of H2O2with range from0.5to6.5mmol/L and, the detection limit was0.05mmol/L. It was a good linear relation between its absorbtion value and its concentrate of glucose with range from0.5to4.5mmol/L, and the detection limit was0.01mmol/L. On the Paper-based microfluidic chip, it was a good linear relation between absorbtion value and concentrate of H2O2with range from0.5to6mmol/L and, the detection limit was0.05mmol/L. It was a good linear relation between its absorbtion value and its concentrate of glucose range from0.5to5mmol/L and the detection limit was0.1mmol/L, and this method has been successfully applied in the glucose detection of serum samples. Part two: Paper-based microfluidic chip’s starch-KI-gelatin-SDS system for glucose detectionThis part based on the Part one that gelatin had a sensitization effect on the starch-potassium iodide-H2O2system, studied the influence of surfactant SDS on chromogenic system. The results showed that after the cationic surfactants, the anionic surfactant, and the nonionic surfactant were added into the starch-potassium iodide-H2O2system, made the chromogenic system’s maximum absorption wavelength moved to the short-wave direction, and starch-potassium iodide chromogenic system’s classical blue color became purple. Sodium dodecyl sulfate was as the model of surfactant in this experiment, combined with the paper-based microfluidic chip technology, through the scanner imaging and Adobe Photoshop software data processing the color results, established a new method which based on a starch-potassium iodide-gelatin system to measure H2O2and glucose, and used for quantitative analysis of glucose in serum. Under the optimized conditions, it was a good linear relation between absorbtion value and concentrate of H2O2with range from0.3to6mmol/L and, the detection limit was0.05mmol/L. It was a good linear relation between its absorbtion value and its concentrate of glucose with range from0.3to6mmol/L, and the detection limit was0.1mmol/L. On the Paper-based microfluidic chip, it was a good linear relation between absorbtion value and concentrate of H2O2with range from0.5to5.5mmol/L and, the detection limit was0.1mmol/L. It was a good linear relation between its absorbtion value and its concentrate of glucose range from1to9mmol/L and the detection limit was0.1mmol/L, and this method has been successfully applied in the glucose detection of serum samples.Part three: Paper-based microfluidic with double enzyme system used to glucose, uric acid, lactic acid detection at the same time This chapter used the ternary double enzyme chromogenic system, namely the glucose oxidase/lactic acid oxidase/uric acid oxidase and hydrogen peroxide oxidase, conbined with the chatacteristic of the paper-based microfluidic, using the scanner imaging and Adobe Photoshop software data processing the color results, implements the glucose, uric acid, lactic acid had a rapid detection at the same time. Results showed that: under the optimum conditions, it was a good linear relation between absorbtion value and concentrate of glucose, uric acid and lactic acid, respectively between0.1~1.4mmol/L,0.1~1.5mmol/L and0.1~1.3mmol/L. Established a paper-based microfluidic chip with a rapid and economic double enzyme chromogenic system, used for establishing a new method for detecting glucose, uric acid, lactic acid ternary system at the same time, this method was rapid, simple and high sensitivity. Provide a scientific experimental basis for patients with diabetes blood glucose concentration of POCT testing. For the treatment of diabetic disease rapid diagnosis, assessment of prognosis judgement and timely control the condition development has very important significance.