The Correlation Of Human Papillomavirus Infection And Nasopharyngeal Carcinoma In Fujian

Objective: Recent reports have implicated oncogenic human papillomavirus (HPV)in nasopharyngeal carcinomas (NPC) of non-endemic regions, especially in Caucasian,but little is known about the prevalence of HPV in NPC in Southern China. The aimof this study was to investigate the infection rate of HPV in NPC from Fujianprovince in Southern China.Subjects and Methods: Samples from70patients with NPC and25patients withchronic inflammation were finally collected. All samples were detected for HPV DNAby polymerase chain reaction (PCR) with the HPV further genotyped by surfaceplasmon resonance (SPR). HPV16/18E6and LMP-1（Latent membrane protein-1）were detected using immunohistochemistry and EBER was detected using in situhybridization.Results: Only two cases of70patients showed evidence of HPV DNA by PCR, Thetwo HPV-positive cases were both non-keratinizing carcinomas with onedifferentiated and being HPV-70in subtype and the other non-differentiated and beingHPV-18in subtype. Both of the two HPV positive cases did not show any evidence ofEBV. The rest of our data showed that57of70NPC samples were detected positivefor EBER, but only25out of70NPC samples were detected positive for LMP-1.Conclusion: Our study indicates that the prevalence of human papillomavirus in NPCpatients of Fujian province in Southern China is low and there is no strong evidenceof HPV and EBV co-infection.