Extraction And Degradation Optimization And The Analysis Of Monosaccharide Composition Of The Polysaccharide From Adenophorae Radix

Adenophorae radix (Nan shashen), a traditional Chinese medicine, is the dry root ofAdenophora tetraphylla (Thunb.) Fisch. or Adenophora stricta Miq. It has been reportedthat the Adenophorae radix contains polysaccharide, triterpenes, β-sitosterol, phospholipids,and other chemical ingredients. Adenophorae radix polysaccharide has a wealth ofbiological activities, such as anti-radiation, immune-regulating, anti-aging, anti-tumor,reduced learning, memory disorder, and so on.Response surface methodology (RSM) aims to achieve the optimum conditions basedon a polynomial equation that fitted between response(s) and factors using the experimentaldata that can be used to estimate the parameters. It can be well applied when a response or aset of responses of interest are influenced by several variables. The purpose of the method isto simultaneously optimize the levels of these variables for obtaining the optimal conditions.This process includes the design of experiment, modeling, date analysis and resultsoptimization.The study optimized the extraction conditions of Adenophorae radix polysaccharide byRSM. The aim of the study is to further save the cost of industrial production and improveits efficiency.The controllable degradation of Adenophorae radix polysaccharide is conducted byacid, enzyme and ultrasonic. It can lay the foundation for the further study ofstructure-activity relationship through the oligosaccharide fragments.The degradation conditions were optimized by orthogonal experimental design and themonosaccharide composition was analyzed by capillary zone electrophoresis (CZE). Itsupported the study of the first level structure and quality control of Adenophorae radixpolysaccharide.