| Objective:Compare80%portal vein ligation to80%hepatectomy model on remaining liverregeneration in rat models.Methods:Experimental Animals: SD rats, healthy male; body weight:200to240g;animals were divided into three groups: Group A:80%portal vein ligation; Group B:85%hepatectomy; Group C: sham operation group. A, B, C groups were of10rats ineach,postoperative survival rate was detected in each group. Six rats were sacrificedat24,48,72and120hours postoperative respectively in all experimental groups, theserum ALT and AST levels and relative liver weight were measured.The expressionof PCNA was detected by immunohistochemistry and the expression of CyclinDl wasexamined by western blotting in group A and BResults:①Survival rate: In Group A, ten rats survived beyond120hours,the survivalrate was83.3%. In Group B, seven rats survived more than120hours, the survivalrate was only58.3%, The survival rate of Group A was significantly higher thanthat of group B(P <0.05). In group C, no rats died at each time point, the survival rateof group C was significantly higher than that of B group (P <0.05), while nosignificant difference was found between group A and group C.②The relative liver weight: The liver regeneration index gradually increased. At24hour, the relative liver weight of group A and B and C were1.32±0.05,1.48±0.03and1.28±0.08, respectively. Relative liver weight of group B was significantlyhigher than group A (P <0.05). At48,72and120hour, the group A’s relative liverweight were2.11±0.10and2.52±0.09and3.28±0.07; the group B’s relative liverweight were1.91±0.08and2.38±0.06and3.17±0.03; the group C’s relative liverweight were1.32±0.07and1.36±0.05and1.38±0.02. Group A was higher than group B at48,72and120hour after surgery (P <0.05). Compared with group C, theliver regeneration index postoperatively in group B was significantly higher at24,48,72and120hour(P<0.01), the liver regeneration index in group A was significantlyhigher at48,72and120hours after operation(P<0.01).③Serum ALT and AST levels: In all groups, the serum ALT and AST levelsincreased rapidly after surgery and reached the peak at24hour, and then began todecline. Compared with group A, the serum ALT and AST levels in group B weresignificantly higher at24,48and72hour (P<0.01) and no significant difference at120hour after operation (P>0.05). Compared with group C, the serum ALT and ASTlevels in group A and group B were significantly higher at24,48and72hour afteroperation(P<0.01), while no significant difference was detected at120hour (P>0.05).④Liver PCNA index: At24hour, the group of B and A’s PCNA index were42.31±1.14and22.14±2.01, respectively. the PCNA index in group B reached thehighest level, which was much higher than that of group A (P<0.01). At48hour, thegroup of A and B’s PCNA index were45.61±1.54and27.48±2.13, respectively.The PCNA index of group A reached the highest value, which was significantlyhigher than that of group B (P<0.01). At72and120hour, the group A’s PCNA indexwere14.72±1.03and4.27±0.09; the group B’s PCNA index were11.68±1.21and3.96±0.11. The PCNA index of Group A was higher than group B at72and120hourafter operation(P <0.05).⑤Liver cyclinD1expression: The expression of CyclinD1increased slightlyafter80%of portal vein ligation and reached the peak at48hour in group A.However, the peak time occurred at24hour after80%hepatectomy in group B. At24hour, the expression of CyclinD1in groups B and A were0.58±0.09and0.21±0.05,compared with group A, the expression of CyclinD1in group B was significantlyhigher at24hour after operation(P<0.01). At48hour, the expression of CyclinD1ingroups B and A were0.23±0.04and0.61±0.11, compared with group B, theexpression of CyclinD1in group A was significantly higher at48hour afteroperation(P<0.01). At72and120hour, the expression of CyclinD1in group B were0.17±0.02and0.05±0.01; the expression of CyclinD1in group A were0.25±0.03 and0.09±0.02,the expression of CyclinD1of group A higher than group B at72and120hours after operation(P <0.05).Conclusion:1. The ligation of80%Liver lobe portal vein led to gradual atrophy of the portalvein blood deprived lobes through hepatocyte apoptosis or ischemic necrosis,whereas the non-ligated lobes underwent compensatory regeneration.80%portal veinligation in rats may be employed as modes for studying liver regeneration.2. Compared with the80%hepatectomy group, the insult of liver cells wereslighter in the80%portal vein ligation group, which was conducive to promote livercell recovery and regeneration postoperative.3. The increasing of the CyclinD1level after portal branch ligation was theindependent factor related to the regeneration in the non-ligated liver lobes.4. The survival rate and liver regeneration of80%portal vein ligation werebetter than80%hepatectomy in this study. |
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