Expression And Significance Of β-catenin In Eutopic And Ectopic Endometrium Stromal Cells Of Ovarian Endometriosis

Objectives：To establish the model of human eutopic and ectopic endometrium cells in vitroculture，and to investigate the expression and the significance of-catenin in eutopicand ectopic endometrium of ovarian endometriosis(OEms).Methods:Division,isolate and culture the eutopic，ectopic endometrium stromal cells ofovarian endometriosis patients and the stromal cells of endometrium in ovarianteratoma as the normal control.Reverse teanscription-polymerase chain reation andwestern-blot were used to detect-catenin expression in those stromal cells.Results:The purities of stromal cells were95%.the IOD values of-catenin mRNA ineutopic and ectopic endometrium of OEms and the normal control were0.672±0.073，0.351±0.029and0.184±0.020respectively,which showed significantly differencesbetween each other groups（F=27.68，P＜0.001）.And the IOD values of-cateninprotein in eutopic and ectopic endometrium of OEms and the normal control were0.803±0.074，0.388±0.044and0.186±0.026respectively,which showed significantlydifferences between each other groups（F=36.83，P＜0.001）.Conclusion:The eutopic and ectopic endometrial stromal cells wre cultured successfully, andthe purities of those stromal cells were95%,its can be used as in vitro cell models ofendometriosis. All of the eutopic,ectopic endometrium and the normal control grouphave the expression of-catenin. The expression of-catenin of the eutopic andectopic endometrium were obviously higher than that of the normal control,theeutopic endometrium is the highest of all those groups.-catenin signal molecule mayplay an important role in the endometriosis ectopic biological characteristics changesand in the ectopic endometrium can complete the "trilogy"(i.e., adhesion, invasionand angiogenesis),and it is closely related with the pathogenesis and development of endometriosis.