The Animal Research Of Platelet-rich?brin (PRF)Guide Bone Regeneration In The Bone Defect Repair

Objective:To investigate and Comparative Study Choukroun platelet-rich fibrin (Choukroun’s platelet-Rich fibrin, Choukroun’s PRF), bio-membrane with bone meal roled in New Zealand white rabbit mandible bone defect at the early reconstruction. We provide theoretical and experimental basis for oral implantology clinical applications and basic research,.Methods:12months old female New Zealand White Rabbit12, weighing3.5kg, adaptive feeding for a week, were randomly divided into two groups (labeled Ⅰ, Ⅱ),(n=6).Experimental animals are weighed,3%sodium pentobarbital injected ear vein anesthesia, ear artery blood placed in5ml blood collection tubes and centrifuge3000r/min centrifugation for12min, let it stand for3-5minutes.Blood is divided into three layers, taking centrifugal test tube in the middle layer, is PRF gel, then place PRF gel to two layers of sterile gauze, gently press into membranous to get PRF membrane.To do an about2cm incision in New Zealand white rabbits beside bilateral mandibular edge, following skin incision, separate the subcutaneous tissue, muscle layer directly to the periosteum.After stripping the periosteum from the mandible edge, creating diameter7mm deep5mm bone defect model.Normal saline, respectively. Group I:12mandibular bone defect randomly use method A:implantation of bone meal covered with the PRF and method B:implantation of bone meal covered with bio-membrane;Group II:12mandibular bone defect randomly use method C:implantation of bone meal and PRF mixtures, and method D:implantation of bone meal.Layered tight suture muscle and skin and given postoperatively to40000units a day gentamicin intramuscular injection, a total of five days.After2weeks,4weeks,8weeks were executed by the marginal ear vein air embolization, after that, gross observation, HE staining and CT bone mineral density testing, analysis results, SPSS13.0software statistical analysis, P<0.05was considered significant differences. Results:12New Zealand white rabbits were no deaths and purulent infection occurs.(1) general observation:After2weeks,4weeks,8weeks, the same observation period of bone defects in bone healing hardness of group A (the PRF cover the bone meal group) and group C (PRF mix bone meal group) is similar,and better than in group B (bio-membrane cover bone meal group) and group D (bone meal group), group B is better than in group D(2) histological observation:after2weeks,4weeks,8weeks, bone in paraffin sections after HE staining new bone formation, and revascularization.Comparing the group A (PRF cover bone meal group) and group C (PRF mix bone meal Organizations and regeneration of new bone vascularized is better than group B (bio-membrane cover bone meal group), group D (bone meal group),The new bone arrangement is better than group B (bio-membrane cover the bone meal group) and group D (bone meal group) rules.(3) CT bone mineral density measurements:After2weeks,4weeks,8weeks after the rabbit mandibular specimens of cone-beam CT scans measured bone defects of bone mineral density values.Average of2weeks:group A,1112.895, Group B921.0125Group C987.855Group D682.7125;4weeks:group A1570.918, groupB,1105.738, group C1612.445, groupD1007.328;8weeks, group A1965.013, groupB1858.21, group C1919.578, group D1844.763.Statistical analysis, no significant difference between the two weeks, four weeks in group A (PRF cover the bone meal group) and group C (PRF group mixed-bone meal);There is difference between group A,group B and group D;8week:Group D (bone meal group) were statistically significant different with the other three groups, there is no significant difference with Group A Group B Group CConclusion:(1) the PRF with bone meal is superior to single use bone meal on guided bone regeneration of bone defects or cover bio-membrane early.(2) the PRF covering bone meal and PRF mixing bone meal effect of guided bone regeneration of bone defects is basically the same.