| Aims: This study to establish the steady and easily replicated models ofType I diabetes mellitus in rats and examine the effects of the5-HT2A/2Creceptor agonist DOI on micturition in the Diabetic Rat.Methods: Female Sprague-Dawley rats (n=16) were divided into twogroups: type I diabetes mellitus (DM) rats (n=8)and age-matchedcontrols(n=8). DM was induced by an intraperitoneal injection ofstreptozotocin (STZ,65mg/kg) after12h of fasting and detailedcystometrogram(CMG) studies was performed8weeks post-injection with10%urethane anesthesia in all rats. The capacity, residual volume, basalbladder pressure,peak bladder pressure, micturition volume and so on weremeasured. The selective5-HT2A antagonist ketanserin was administered aftereach DOI dose-response curve. All drugs were administered intravenously.Results: Compared to controls, comprehensive urodynamic studiesdemonstrated DM rats had a higher bladder capacity and residual volume, anda markedly reduced voiding efficiency. In DM rats，DOI (0.01to0.3mg/kg)induced significant dose dependent decreases in residual volume， andincreases in micturition volume,resulting in increased voiding efficiency. CMG measurements showed a dose-dependent increase in high-frequencyoscillations(HFOs) activity, evident as an increased duration of HFOs pervoiding, which correlated with the improved voiding efficiency. Ketanserin(0.1mg/kg) partially or completely reversed the DOI induced changes.control group showed few significant changes in cystometric variable.Conclusions: Established rat model of DM is steady and easily toreplicate by effective care and convenient methods. The bladder voidingefficiency was decreased in DM rats.5-HT2A receptor agonist can enhanceHFOs activity and ameliorates voiding efficiency, thereby5-HT2A receptoragonist may represent a new strategy to improve voiding efficiency after DMin experimental studies. |
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