The Comparative Study On The Activity Of The Grain Adipose Tissue In Different Cryopreservation Condition

Background: With the development of plastic surgery, autologous fattransplantation has quite won the heart of plastic surgeon and the people who want to bebeautiful by medical methods, as the result of its abundance, easy manipulation,minimal harvest trauma, low cost, good compatibility, repeatable harvest, and otheradvantages. Adipose tissue transplantation has become a common used method in softtissue reconstruction. However, the main obstacle to achieve favorable outcome in theautologous fat transplantation is the unpredictable and high rate of absorption in therecipient site. Nowadays, adipose aspirates can be used only by immediate autologousfat grafting at the same time with liposuction. The superfluous adipose aspiratesobtained from the procedure are usually discarded. Furthermore, repeated liposuctionincreases the mental pressure and clinical risk of both patients and operating surgeon.Adipose tissue can be preserved in vitro, and the autologous fat transplantation patterncan be changed from the repeated liposuction to the usage as you like, as can benefitboth to patients and surgeons.Objective: By biochemical detection of glucose transplantation of adipose cell migration, and inverted microscope observation of trypan blue staining of adipose cell’spercentage changes, the effects on the adipose cell activity in different conditions ofcryopreservation are evaluated to provide a reference for the choice of suitableconditions of fatty tissue in vitro storage and for further research and clinicalapplications.Methods: Adipose tissue derived from the liposuction patients in the department ofplastic surgery, General Hospital of Shenyang Military Area Command. Adipose tissuewas harvested with conventional liposuction and then collected f-rom the resultingmiddle layer after centrifugation. The fat samples were divided into three groups:control group (fresh fat group), experimental group1(physiological saline group), andexperimental group2(trehalose group), the trypan blue staining and glucose transfermethod were used to test the different number of activity cells. The experimentalgroup were under three different conditions of frozen of-18℃fat (ordinaryrefrigerator),-80℃(deep freezer),-196℃(liquid nitrogen) respectively. After3monthsfrozen, to test the adipose tissue activity detection as in the same way as theexperimental group. And the results will be analyzed by the SNK version statisticssoftware.Results:1. The viability of fat cell is the highest when trehalose group is frozenat196℃, reaching73%of the viability before freezing. And it is less than50%whenfrozen at-18℃.2. In the same freezing temperature, trehalose group’s fat cell activity iscloser to the fresh cell group than the saline group.3. With the same additive, thefreezing effect of-196℃(liquid nitrogen) is better than that of-18℃(ordinaryrefrigerator) and-80℃(deep low temperature frozen refrigerator).Conclusion:1.Adipose tissue, with additive of trehalose, will has the bestcryopreservation effect under the conditions of-196℃(liquid nitrogen).2. Trehalose,as cryoprotectant, can effectively prevent frozen cells from low temperature injury instoring process, and can contribute to adipose tissue’s long-term preservation.3.In thelong-term storage, the cell activity will be maintained better at the temperature of-196℃(liquid nitrogen) which is best temperature of frozen.4. The activity of frozen fat cell is still not as well as the activity of fresh fat cells; it will need moreanimal and clinical study to apply the human adipose cells cryopreservation in clinicalapplication.