| The obligate intracellular bacterium Chlamydia trachomatis (C.t) parasites in eukaryotes and is the most common pathogens of sexually transmitted disease all around the world currently. The mild and concealed symptoms in early stage of the diseases that infected by C.t made it easy to be ignored, spread and chronic persistent infection. Long term infection can cause a series of serious complications and sequelae, including endometritis, tubal infertility, epididymitis, prostatitis, and in some severe cases can lead to cervical cancer or pelvic cancer in women, penis cancer and rectal cancer in male, and squamous cell carcinoma. Different serotypes of Chlamydia trachomatis can lead to different types of diseases, trachoma (serotypes A-C) and sexually transmitted serotypes D-K cause trachoma and uro genital tract infection, while Venereal lymphogranuloma serotype L are related to lyphogranuloma venereum (LGV). LGV is mainly spread by sexual contact and we generally divide the course of disease into three stages. A transient vesicular damage in genital area and repeated local lymph nodes are primary clinical manifestations of LGV, elephantiasis and rectal stricture may occur late or untreated, a minority of patients can be complicated by cancer. These diseases now in China are relatively rare.Background and Objective:The domestic case of a patient diagnosed with lymphogranuloma venereum complicated by cutaneous squamous cell carcinoma in metaphase has been found in our transmitted diseases research institute. LGV combined with cancer can be seen in the phage III stage with long-term repeated lymphadenopathy has only few reports, and is considered with scarring and local stimulation. In this case, the cancer occurs in the duration of interim (bubo period), The glans penis frenulum secretion and bilateral groin puncture fluid chlamydial endogenous plasmid PCR were positive, omp-1restriction fragment length polymorphism analysis (RFLP) shows type L3, suggesting that L3type of Chlamydia trachomatis infection may induce cancer easier.Therefore, in this paper we put this case as an entry point, detect C.t infection through the patient’s cancerous tissue and secretions, and use clinical specimens and LGV L3standard strains to investigate the correlation between LGV and squamous cell carcinoma in vitro. To explore the effect of Chlamydia trachomatis LGV serovars on proliferation activity of cells.Methods:1.Presenting a case of LGV complicated with squamous cell carcinoma found in2010at Tianjin Medical University sexually transmitted disease Institute.2. Obtain the inguinal mass and inguinal squamous cell carcinoma biopsy specimens to detect the C.t infection by immunohistochemistry. Obtain the purulent secretions from inguinal lymph nodes to detect the C.t infection by C.t culture with McCoy cells and Hela cells respectively, and PCR method.3. Use clinical specimens from case patient and C.t lymphogranuloma venereum L3serotype standard strains to intervene McCoy cells and Hela cells respectively to detect cell proliferation by cell counting and MTT assay.Results:1. Positive results of C.t detection by Immunohistochemistry are received in tissues of inguinal mass and inguinal squamous cell carcinoma.2. C.t culture with purulent secretions from patient’s inguinal enlarged lymph nodes were succeed within Hela cells, while failed without detection of inclusion body in McCoy cells. PCR test are negative.3. MTT and cell counting results showed that clinical specimens and C.t lymphogranuloma venereum L3serotype standard strains could promote cell proliferation, and the latter showed a significantly promoting effect on Hela cells proliferation.Conclusions:1. Disease of this patient may develop into squamous cell carcinoma via LGV Chlamydia trachomatis L3serotypes infection.2. Chlamydia trachomatis serovar L3can significantly promote cell proliferation, suggesting its carcinogenic effect. |
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