Invigorates The Kidney Fills In Fine Method The Influence Which Leads To Patients With Premature Ovarian Failure Clinical Observation And The Mouse Ovary Growth Differentiation Factor-9Signal Transduction

ObjectiveThe observation Chinese medicine invigorates the kidney fills in the fine method treatment premature ovarian failure the curative effect, and to explore the treatment and prevention of premature ovarian failure. The research invigorates the kidney fills in the fine Chinese native medicine the influence which leads to the immunity premature ovarian failure mouse ovary growth differentiation factor-9signal transduction, further explains invigorates the kidney fills in the fine method treatment immunity premature ovarian failure the mechanism.MethodClinical research part of the collection in November2011to March2014in the first affiliated hospital of guangzhou university of TCM gynecology clinic, in line with the diagnostic criteria of25cases of patients with premature ovarian failure. At the same time, according to selection of di-minished ovarian reserve by21patients as control group. Fill with Invigorates the kidney fills in fine method,6months of treatment were observed before and after treatment serum FSH, LH, E2level change and major clinical symptoms improve the situation. Experimental research on some selected BALB/C8weeks of84female mice, were randomly divided into7groups: POF model, POF model group4weeks8weeks group,4weeks group, Zuogui pill Zuogui pill8weeks group,4weeks group, diethylstilbestrol diethylstilbestrol8weeks group, blank control group, establishes the immunity premature ovarian failure animal model with the Zona Pellucida3(ZP3) abdominal cavity and subcutaneous multi-point injection immunity in mice(Immunity dosage is0.15ml, It contains0.03mg ZP3). and blank group for the same amount of distilled water. After the success of the building, respectively using Zuogui pill and diethylstilbestrol lavage treatment, continuous lavage four weeks or eight weeks, and in the corresponding period of ovarian tissue in mice. And using Western blot method to detect GDF-9, Smad2protein expression level in ovarian tissue.Results1.25cases of POF women aging disease mainly concentrated in26to30years old age, accounted for40%, lowest proportion of20to25years old age, accounting for16%.2. Clinical observation of25cases of POF women usually have irritability accounted for64%, had depression anxiety accounted for28%.3.25cases of menstrual POF patients for more than6months (amenorrhoea) were15cases, accounting for60%, menstrual block4-6months (late period) of the10cases, accounting for40%. After treatment for Invigorates the kidney fills in fine method, amenorrhea and menstruation late points before and after treatment difference had statistical significance (P<0.05), the period of the total effective rate was64%.4. The main clinical symptoms of POF patients’ are upset, irritability, insomnia, hot flushes the sweat out, sexual intercourse pain, muscle joint pain. After treatment for kidney fill in essence,hectic fever and sweating, the main symptoms frequency decreases, and the hot flashes khan, upset, irritability, depression, obviously improved the symptom such as insomnia, symptom integral compared with before treatment difference was statistically significant (P<0.05).5. POF patients, the serum level of FSH by Invigorates the kidney fills in fine method decreased after treatment, there was statistically significant difference before and after treatment (P<0.05); the level of LH after the treatment has decreased, but no significant differences in E2levels before and after treatment; after the treatment has increased, but no significant difference before and after treatment (P>0.05).6. The premature ovarian failure group model mice vaginal exfoliated cells smear mainly for a large number of white blood cells and vaginal exfoliated cells smear a small amount of epithelial cells, give priority to with estrogen interphase. Of mice model of premature ovarian failure The premature ovarian failure group GDF-9in the ovarian tissue and its signal transduction protein Smad2expression was lower than those of blank group.7. Zuogui pill group of GDF-9protein in the ovarian tissue in mice and its signal transduction protein expression Smad2higher than model group.Conclusion1. Premature ovarian failure patients is closely related to the modern factors, but also with the irregular of diet, overwork disorders, productive, pelvic surgery and other factors.2. Invigorates the kidney fills in fine method to treat patients with premature ovarian has certain curative effect, can improve the menstrual status and clinical symptoms, can reduce the patients with high serum FSH, LH levels increased, low E2levels.3. It was Successed with ZP3immunogenicity POF mice model. Autoimmune premature ovarian disease may be collected with GDF-9protein expression in ovarian tissue weaken, and may be affected by the GDF-9/Smad2signal transduction pathway, accelerates the follicle growth obstacle, follicles depletion, accelerating follicular atresia, that cause premature ovarian failure.4.Kidney fill pure traditional Chinese medicine (TCM) Zuogui pill can improve mice GDF-9protein expression in ovarian, may through regulating the GDF-9/Smad2signal transduction play the adjustment role of follicles, thus achieved promotes ovulation, adjust the action of the ovarian function.