Protective Effects Of Polysaccharides From Boschniakia Rossica On Oxidative Damage Of Liver Cells

Objective:To study the protective effect and mechanism of polys accharides from Boschniakia rossica (BRPS) on oxidative damage of1iver cellsMethods:H2O2induced liver injury model, using toxicological eff ects of the tetrazolium salt colorimetric (MTT) assay BRPS in liver c ells; The lactate dehydrogenase (LDH), alanine aminotransf erase (ALT) and aspartate aminotransferase (AST) activity, cell superoxide dismutas e (SOD) activity and glutathione (GSH), malondialdehyde aldehyde (M DA) content levels of the supernatant were measured Western blot an alysis Nrf2, COX-2, iNOS and microsomes of HO-1and other therape utic target protein expression and cell extracellular signal-regulated ki nase (ERK), c-Jun N-terminal kinase (JNK), p38mitogen-activated pro tein kinase (p38) and the nucleus of nuclear transcription factor-κB (NF-κB) expression and its phosphorylated form of proteinsResults:BRPS pretreated with different concentrations, compared with the model group, BRPS can significantly improve cell survival r educing liver cell culture supernatant LDH, ALT and AST levels(P<0.05); Reduce intracellular levels MDA (P<0.05), the increased activity of SOD and GSH content (P<0.05) Western results show that compar ed with the normal group, expressions of Nrf2HO-1and iNOS are i ncreased in model group Compared with the model group, the protein expressions of Nrf2, HO-1, iNOS were significantly reduced (P<0.05) in medicated group Compared with the normal group, ERK phosphate JNK and NF-kB protein expressions are increased significantly (P<0.05) in model group Compared with the model group, the expression of t he phosphorylation of JNK, ERK and NF-κB proteins were decreased obviously The expression of p-p38and COX-2had no significant cha nges in medicated groupConclusion:BRPS has obvious protective effect in the liver cells from oxidative injury induced by H2O2, could significantly inhibit H2O2injury to liver cells Its effect may be related to increased antioxi dant capacity, inhibition of ERK, JNK and the regulation of NF-κB a ctivation...