| Background and objectT helper17cells induced inflammation and regulatory T cell induced immunological tolerance rival to each other in function and the process of differentiation. They keep balance in normal state to maintain the stability of immunization in human body, but excessive expression of some cells factors in dysfunction and pathological condition affect the differentiation structure of naive T cells, which also might promote Th17cells differentiation and imbalance of Treg/Th17to induce a series of inflammation. The mechanisms to treat UC (ulcerative colitis) effectively with (KD)Kuijieling Decotion that clear heat and fortify the spleen and activate blood will be further discussed in this study. It will start with Treg/Th17in the sources of effector T cells activation, discussing the mechanisms of balance conversion in Treg/Th17regulated by KD, which demonstrates the deeply mechanism to cure UC with KD.Method60SD rats were randomly divided into a blank control group, model control group, high-does Kuijieling, medium-does Kuijieling, low-does Kuijieling, SASP group. To make UC model rats induced by TNBS and observe the its condition. KD will be intaked after three-days UC model. The rats will be sacrificed and scraped their colonic mucosa to exam in pathology after consective ten-days oral administration. The proportion and percentage of Treg and Th107cells in CD4+T cells in peripheral blood will be detectded by flow cytometry, the content of TGF-β1and IL-6in plasma will be examined by E1ISA, the expression of specific transcription factors Foxp3affected the Treg cells differentiation in colonic mucosa will be observed by Real-time PCR Result1. The Observationon on Pharmacological effect:Compared to model group, the damage in colonic mucosa had been improved greatly in high-does Kuijieling and SASP group.2. The effect on Treg/Th17in peripheral blood:The content of CD4+Foxp3+Treg cells in peripheral blood in model group was significantly lower than blank control group (P<0.01). After the treatment with KD in high, medium and low dosage and SASP, the content of CD4+Foxp3+Treg cells were5.04±0.76%,4.57±1.02%、4.47±0.47%、5.28±0.56%respectively in peripheral blood. Which were greatly higher the model group (P<0.01;P<0.05;P<0.01;P<0.01);The content of CD4+IL-17A+Th17cells in peripheral blood in model group was significantly higher than blank control group (P<0.05). After the treatment with KD in high, medium and low dosage and SASP, the content of CD4+IL-17A+Thl7cells were1.45±0.45%、1.69±0.35%、2.83±1.01%、1.67±0.31%respectively, KD in high,medium group and SASP group were greatly lower the model group(P<0.01;P<0.01;P<0.01). The proportion of Treg/Th17in the model group was lower than the blank control group. After the treatment with KD in high, medium and low dosage and SASP, the proportion of Treg/Th17in peripheral blood were3.69±0.92、2.77±0.69、1.75±0.58、3.28±0.79respectively, Which is obiviously higher than the model group(P<0.01; P<0.01; P<0.05; P<0.01).3. The effect on TGF-β1and IL-6in plasma:The content of TGF-β1in plasma in the model group was438.87±32.07(pg/ml), which is greatly lower than the blank control group (649.08±136.22)(P<0.01). After the treatment with KD in high,medium and low dosage and SASP, The content of TGF-β1in plasma in the KD in high, medium and low group and SASP group were540.72±99.09、587.34±78.60、511.02±59.68、587.74±87.58respectively, which are obiviously higher than the model group (P<0.01). The content of IL-6in plasma in the model group was61.80±4.15(pg/ml), which is greatly higher than the blank control group (55.44±4.41)(P<0.01). After the treatment with KD in high, medium and low dosage and SASP, The content of IL-6in plasma in the KD in high, medium and low group and SASP group were56.72±6.53、54.82±5.67、57.73±7.30、52.72±8.44respectively, the medium-dose group and SASP group is obiviously lower than the model group (P<0.01; P<0.01).4. The effect on expression quantity of Foxp3in colonic mucosa:The expression quantity of specific transcription factors Foxp3in colonic mucosa in model group was0.81±0.23, which was significantly lower than the blank control group (1.36±0.34). After the treatment with KD in high.medium and low dosage and SASP, the expression quantity of Foxp3were1.24±0.43%、1.21±0.53、1.79±0.67、1.98±0.73respectively, the high-dose group、low-dose group and SASP group which were greatly higher than the model group (P<0.01; P<0.05; P<0.01).Conclusion1. Obivious effect existing for KD to cure UC rats induced by TNBS, which perhaps is related to the higher proportion of CD4+Foxp3+Treg cells and the lower proportion of CD4+IL-17A+Th17cells in peripheral blood and theirs balance.2. The KD might have effect on up-regulating and down-regulating the content of cytokines TGF-β1and IL-6respectively, promoting the differentiation of Treg cell, strengthening Treg function and repressing the differentiation of Th17cell to regulate the balance of Treg/Th17in vivo so as to recover the normal immunological state and alleviate the inflammation.3. For the significant effect of KD existing, the transcription factors Foxp3promoted the Treg cells differentiation and its function were up-regulated, the differentiation of Th17cells were repressed so as to recover the balance of Treg/Th17in vivo. |
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