Effect Of Vitamin E On Fetal Heart Apoptosis Of Gestational Diabetes Mellitus Rat

Objective: To investigate the effects of oral vitamin E and folic acid on thecardiomyodial apoptosis of offspring in an experimental model of hyperglycaemiapregnant rats. Methods:50clean pregnant rats were randomly divided into5group A、B、C、D、E. Except control group， group B、C、D、E were injectedintraperitoneally35mg/kg streptozotocin (STZ)on the third day of pregnancy, thenormal control group were injected with the equal dose of citrate buffer. From the5th day of pregnancy, C、E group were intragastriced with folic acid0.4mg/kg；D、E group were intragastriced with vitamin E50mg/kg；and A、B group withsame volume of distilled water for7days. After nature birth, myocardialpathological changes were observed for the neonatal rats under light microscope;Immunohistochemical and western-blot were used to detect apoptosis in the heartof fetus. Results:1.The blood glucose of pregnant rats in the5groups showed no difference before molding (p>0.05).There is significant difference between groupB and A、group C and A、group D and A、group E and A（p<0.01）, but nodifference among group B、C、D、E(p>0.05).2.Under light microscope， themyocardial tissue in group B、C、D、E showed different degree damage, necrosis、edema， the myocardial fibers showed disorder and inflammatory cellsinfiltration.3.In the fetal hearts, the mean OD value of caspase-3in5groups hadsignificant deviation (p<0.01).4.Among5groups, the grey value of caspase-3protein expression showed significant difference of fetal rats cardiacmyocytes(p<0.01). Conclusions: Gestational diabetes mellitus can cause fetalcardiac myocytes apoptosis, vitamin E and folic acid supplementation duringpregnancy have protective effect on the apoptosis of fetal cardiac myocytes, folicacid has an advantage over vitamin E to inhibit fetal cardiac myocytesapoptosis,supplementation of vitamin E and folic acid meanwhile is superior tosupply alone one of them.