In Vitro Evaluate The Expression And Differentiation Capacity Of COX-2Silencing In Human Bone Marrow-derived Mesenchymal Stem Cells

Background At present, sliencing of COX-2in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) was at preliminary stage.Objective This study was to construct the lentivirus vector, then observe the expression and differentiation capacity of COX-2silencing in human bone marrow-derived mesenchymal stem cells.Methods In the present study, the recombinant lentiviral vectors carrying sliencing of COX-2gene and green fluorescent protein were constructed with recombinant lentiviral technology, and then we knocked down COX-2expression in hBMSCs through lentivirus infection in vitro (Lenti-ShCOX-2group). The hBMSCs transfected with single lentivirals (Lenti-Shcontrol group). Single hBMSCs as control (untreat group). At7days after trasfection, the total RNA and protein were extracted from each group for detection. The differentiation capacity of these cells were assessed in vitro.Results and Conclusion After transfected with lentivirus vector for3days, we could observed the green fluorescence of hBMSCs with COX-2sliencing under fluorescence microscope, the transfection efficiency was over90%in Lenti-Shcontrol-2group,85%in Lenti-ShCOX-2group. We confirmed the successful down-regulation of COX-2at the mRNA and protein levels in hBMSCs through lentivirus infection.lentivirus infection did not inhibit the differentiation capacity of hBMSCs.