Effect Of Low Concentrations Of Nicotine On Soft Tissue Defect Repairment Of Rat Hard Palate

Background and Objective: The maxillofacial tissues,which were more exposedand less protection than the rest of the body，had become one of the most common site oftrauma. Trauma of the maxillofacial tissues often occurred in34%patients who hadsystemic trauma resulting from incidence of traffic accidents and other accidents[1]. Theskin and mucous damage, or jaw fracture, caused by oral and maxillofacial trauma, oftenlead to varying degrees of dysfunction in language, swallowing and chewing, and lead tomaxillofacial deformities. The dysfunction and deformities would affect the patient’slife and social activities, and even cause great physical and psychological disorders. Solooking for a quick, perfect way to promote physiological repair of maxillofacial traumabecomes a hot research scholars.Exogenous growth factors can promote wound healing and has been usedclinically. But the exogenous growth factors are expensive and can be metabolism fast,moreover they are inconvenient to be use in the oral mucosa and mandibular fracturetrauma. So using exogenous growth factors in treating maxillofacial trauma on clinical islimited. Nicotine is one of the toxic components of cigarettes. Many studies suggest that nicotine has a negative impact on wound healing, mainly through inhibition ofkeratinocytes, proliferation or migration of fibroblasts and macrophages, increasingtissues blood vessels, reducing nutrient blood flow and thus delayed wound healingmechanisms[2-5]. However, the latest series of studies found that low concentrations ofnicotine could promote angiogenesis, accelerating skin wound healing[6-8]. Whether lowconcentrations of nicotine in the maxillofacial soft tissue, especially in the oral mucosa,play a role in wound repair is unclear. Nicotine has the advantage of low prices. Thisexperiment will initially observe its effect on oral soft tissue injury and repair of the hardpalate by preparing a low concentration of nicotine gel release agent.To looking for newideas and methods for clinically accelerating wound healing of oral mucosa with a safe,convenient, inexpensive wayMethods: A3mm diameter circular soft tissue defects was produced in the centre ofhard palate of125wistar rats. After the operation, animals were randomly divided intothe control group, the gel group and low concentration of nicotine with gel group. Ratswere sacrificed at3,7,10and14day postsurgery. The wound healing was contrastedthrough haematoxylin-eosin (HE) staining and image measurement between each groups.Results: On the third day of postoperative, each group had no significant difference; the7th and10th day groups of low concentrations of nicotine with gel healed faster than thegel group and control group; however there was no significant difference among thegroups on14th days. Conclusion: Low concentrations of nicotine may promote themucosa defects repairmen of rat hard palate.