Effects Of Lemongrass Essential Oil Against Benzo[a]Pyrene Induced Oxidative Stress And DNA Damage In Human Embryonic Lung Fibroblast Cells

Objective:To detecte the contents of superoxide dismutase (SOD), catalase(CAT), malondialdehyde(MDA),8-hydroxy-deoxyguanosine(8-OHdG), benzo[a]pyrene(BaP),3-hydroxy-benzo[a]pyrene(3-OHBaP) and benzo[a]pyrene diolepoxide-DNA adducts (BPDE-DNA) which lemongrass essential oil(LEO) and BaP exposure in human embryonic lung fibroblast(HELF) cells and evaluate the effects of LEO against BaP induced oxidative stress and DNA damage. We will preliminary explore whether LEO become a new source of lung cancer chemoprevention.Method:The HELF cells were selected as target cells using a completely randomized design in vitro. Set0.5%,1.0%and2.5%LEO dose group and25μM BaP dose group,0.1%dimethyl sulfoxide (DMSO) as solvent control. Experimental were divided into four groups:①Solvent control group;②BaP group;③LEO group;④LEO and BaP groups:simultaneous administration of LEO with BaP for24hours. After treated, cells were collected in-80℃. The study reported that the chemical identification of LEO was accomplished by GC/MS analyses. Subsequently, the cell viability of HELF cells was measured by MTT assay and the levels of SOD, CAT, MDA and8-OHdG in HELF cells were analyzed through assay kit respectively. Last, we determined the contents of BaP,3-OHBaP and BPDE-DNA in cells by high performance liquid chromatography with fluorescence detection (HPLC/FLD).Results:(1) The main constituent of the LEO were citral, the relative content of which are neral (37.45%) and geranial (31.33%).(2) Exposure to25uM BaP for24hours caused a sharp decrease (P≤0.01) in cell viability. Yet a significant change was observed that0.5%concentrations of LEO prevent BaP-induced the loss of cell viability (P≤0.01). A single dose of BaP significantly decreased (P≤0.01) the activities of SOD, CAT and elevated (P≤0.01) the level of MDA,8-OHdG compared to the control group. However, Simultaneous administration of LEO with BaP for24hours elevated (P≤0.01) the levels of SOD, CAT and also reduced (P≤0.01) level of MDA,8-OHdG compared to the BaP-treated group.(3) The test method of HPLC/FLD can effectively detect BaP. However the contents of3-OHBaP and BPDE-DNA have no found in cells. Simultaneous administration of LEO with BaP increased the content of BaP compared to BaP-treated group.Conclusion:LEO can increase BaP-induced cell viability, decrease oxidative stress and DNA damage in HELF cells. Our study concludes that LEO is a good antioxidant and have protect effective in combating BaP induced oxidative stress and DNA damage. So, we believe that LEO will be a promising source in future lung cancer chemoprevention.