Effect Of Light Concentration Nifedipine On The Expression Of Bcl-2, Bax And P53in HGECs

Objective This study investigated the effect of minimal level nifedipine (NIF) on transcription of bcl-2,Bax and P53in human gingival epithelialcells (HGECs) in vitro and to study the poseblepathogenesis of epithelial thickening in drug-induced gingival overgrowth(DGO).Methods The gingival tissues obtained from periodontal surgeries were digested with enzyme and HGECs were cultured in vitro; HGECs were identified by immuno-histochemistry; Bcl-2mRNA levels were quantitated by Real-time PCR24hours after cells stimulated by NIF with different concentration (0μg/ml,0.25μg/ml,0.5μg/ml,1.0μg/ml,1.5μg/ml,3.0μg/ml), inwhich0μg/ml NIF as blank control. The datas were analyzed by one-way ANOVA using SPSS11.0software package.Results HGECs cultured in vitro showed keratin positive signal and vimentin negative signal; the level of Bcl-2%、Bax andP53mRNA increased with NIF1.5μg/ml compared with3.0μg/ml group, after decreased in all of the target groups compared with blank control (P<0.05).Conclusion NIF regulates the level of Bcl-2,Bax,and P53mRNA in minimal level. It regular the level of Bax through modifying the P53level.