The Study Of Relationship Between T Cell Subtype Drifting Change And Glycometabolism In Type-â…¡ Diabetes Patients With Phlegm-wet Constitution

Objective:This study focuses on type-II diabetes patients with phlegm-wet constitution, to observe drifting change of peripheral CD4+helper T cell subtypes, as a result to investigate the relationship between cellular immunity properties and glycometabolism relative index in type-II diabetes patients with phlegm-wet constitution. In addition, from a cellular immunity perspective, this study is trying to interpret that there might be a greater change in cellular immunity change in type-II diabetes with phlegm-wet constitution, which might have a significant meaning in understanding of the etiology and pathogenesis, as a result to provide for theoretical references for treating diabetes with Chinese medicine.Methods:65patients from medical examination center and endocrinology clinic in Guangdong Provincial Hospital between December2013to March2014were recruited, with age ranged from18-70years old. In accordance with the ADA(2010) diabetes diagnostic standard and phlegm-wet constitution diagnostic standard from<Somatology in Chinese medicine>, all patients were allocated into control group with20cases and T2DM phlegm-wet constitution group with23cases and T2DM non-phlegm-wet constitution group with22cases, respectively. This study collected objectives’ gender, age, BMI, waist line, as well as biomedical indices. This study also used flow cytometry technology to test peripheral T cell subtypes Thl, Th2and Thl/Th2proportion. All data were processed in epidata3.1software and then forwarded to SPSS to be further processed in SPSS20.0. Statistical population was expressed by mean±standard deviation, and×2test was used in qualitative data, whereas, t-test for quantitative data. Independent sample t-test was used in inter-group qualitative data. Multi-group data comparison was processed with single analysis of variance, taken significance level α=0.05by ANOVA, P>0.05was no statistically significant difference, P<0.05was considered statistically different.Result:1.3groups of gender, age were processed by×2chi-square test, the results showed no statistical significance (P>0.05), baseline data for each group showed good comparability.2. T2DM phlegm-wet group and T2DM group of non-phlegm-wet group comparison: Waistline, BMI, abdominal fat thickness, TC, TG, LDL-C, FBG, Th1, Th1/Th2of T2DM phlegm-wet group were significantly higher than non-phlegm-wet group, the difference was statistically significant (P<0.01); HDL-C of T2DM phlegm-wet group was significantly lower than the T2DM non-phlegm-wet group, the difference was statistically significant (P<0.01); Th2in the two groups had no statistically significance (P>0.05)3. T2DM phlegm-wet group and control group comparison:Waistline, BMI, abdominal fat thickness, TC, TG, LDL-C, FBG, HbAlc, Thl, Th2, Thl/Th2of T2DM phlegm-wet group were significantly higher than the control group, and the difference was statistically significant (P<0.01); HDL-C of T2DM phlegm-wet group was significantly lower than the control group, the difference was statistically significant (P<0.01).4. T2DM non-phlegm-wet group and control group comparison:Waist line, BMI, TC, TG, FBG, HbAlc, Th1, Th2, Th1/Th2of T2DM non-phlegm-wet group were significantly higher than the control group, the difference was statistically significant (P<0.01); T2DM phlegm-wet group and normal control group of abdominal fat thickness, LDL-C, HDL-C, the difference was not statistically significant (P>0.05).Conclusion:1. Type2diabetes can lead to lipid disorders, patients with phlegm-wet constitution had more prominent dyslipidemia in patients with non-phlegm-wet constitution.2. Type2diabetes patients had abnormal cellular immunity, and patients with phlegm-wet constitution were more predisposed to T cell subtype disorder, and T cell subtype disorder had relationship with elevated blood sugar and HbAlc.