The Methylation Profile Of The Promoter Cpg Islands Of Srd5a2in Hepatocellular Carcinoma

The aberrant status of DNA methylation contributes pathlogically to the initiation and development of the major several diseases, incuding cancer. The cancer associated changes goes two ways:the global hypomethylation and local hypermethylation of the promoter CpG island of the tumor suppressor genes. In this study, the methylation status of the promoter CpG island of of SRD5A2has been determined in12hepatocarcinoma cell lines,6healthy liver tissues and20liver cancer (and the neighboring non-cancerous) tissues. The methylation specific PCR analysis was used.The discovery of human cancer biomarkers for tumor subtypes precise classification and the development of clinical prognosis methods is very important. Although, for cancer diagnosis and prognosis of the genetic map of map (SNPS, LOH, mutation) and expression spectrum (mRNA and protein expression spectrum) biomarkers has been large-scale analysis, but the use of epigenetics fingerprint analyses early diagnosis and clinical prognosis of the tumor showed a greater potential, this potential greatly promoted the DNA methylation prognosis biomarkers for cancer research.In addition, short experiment period, simple operation, low cost. Its disadvantage is to know in advance for the segment of DNA sequence, need two set of known key CpG site design upstream and downstream primers, and the site must be fully methylation or no methylation. Can completely satisfy these conditions, in fact, less number of genes, this method has very obvious "selective" genes. At the same time it can clear whether methylation; If you want a quantitative, the need to use other methods to further testing.SRD5A2Genes can in cells that are sensitive to testosterone, such as the prostate, liver high level coding SRD5A2microsomal protein in the cell. Conclusions are as follows:SRD5A2was found homozygously methylated in8of hepatocarcinoma cell lines:Hep-3B, Hep-G2, SMMC-7721, SK-Hep-1, YY-8103, BEL-7404, BEL-7405and QGY-7701, heterozygously methylated in cell line QGY-7703and homozygously demethylated in cell lines of Focus and PLC. The analysis of BEL-7402failed. Its homozygous methylated state was unexpected to be found in1case of the healthy liver tissue while the rest homozygously unmethylated. Its methylated state was found is30%(6/20) of the cancer and10%(2/20) in the adjacent normal tissues.