| Objective:To research the connection of immune inflammatory disorder and immune inflammatory thrombophilia in the collagen type II-induced arthritis (CIA) rats,and explore the regulatory effect of umbilical cord mesenchymal stem cells (UC-MSCs) homing on immune inflammatory disorder and thrombophilia. To research the impact of inflammatory disorder to the prothrombotic in the CIA rats, and UC-MSCs homing regulate the immune inflammatory disorders and thrombophilia status.Methods:144(Sprague-Dawley Rats) SD rats were randomly divided into control group (group C), model group (group M), UC-MSCs treating group which labled by SPION (group SU), the group added antagonist AMD3100to labeled UC-MSCs(group ASU).Except for group C, the other rats were established model of CIA. After modeling is completed, the treating group were injected the suspension of UC-MSCs, tagged UC-MSCs and tagged UC-MSCs co-culture with antagonist via the tail vein respectively. After the rats were injected UC-MSCs were sacrificed in the first,third,fifth week,and we extract the abdominal cavity venous blood and remove the fresh knee tissue.The concentration ofInterleukin-10(IL-10),Interleukin-6(IL-6),Tumor necrosis factor-a(TNF-a), Interferon-y(IFN-γ) and tissue factor(TF), von Willebrand factor(VWF), D-dimer(D-D), Fibrinogen(FIB) in sera of rats from each group were determined with the enzyme-linked immunosorbent assay (ELISA) and the expression of IL-10,IL-6,TNF-a, IFN-yin knees cartilage of rats from each group was evaluated by western blotting.Results:1CIA rats joint swelling and ulceration after successful modeling, their activity were limited, the levels of pro-inflammatory cytokines IL-6, TNF-α, IFN-γ in group M were significantly higher than the control group since the first week (P<0.05), and prethrombotic indicators TF, VWF were significantly difference compared with the group C (P<0.05).2. After given UC-MSCs treatment,the levels of pro-inflammatory cytokines IL-6, TNF-α, IFN-γ in group SU was conspicuously reduced compared with group M since the first week.The decrease had statistical differences compared with the group M in the fifth week,(P<0.05).The levels of anti-inflammatory cytokine IL-10increased from the first week when compared with group M were statistically significant in the fifth week(P<0.05).3After adding antagonist, the levels of pro-inflammatory cytokines IL-6, TNF-a, IFN-y and anti-inflammatory cytokine IL-10in group ASU were similar to group M (P>0.05). The levels of TF, VWF, D-D, FIB were no significal differences compared with the group M (P>0.05).Conclusion:1.The immune inflammatory disorders in CIA rats play important effect on the developing immune inflammatory thrombophilia.2.UC-MSCs can repair inflammatory disorders and thrombophilia in CIA rats by homing to injured tissue.3.The immune repair of UC-MSCs by homing can suppressed by AMD3100. |
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