Study On Technology For Extracting Lignans And Polysaccharides From The Fruit Of Schisandra Chinensis Baill

Schisandra chinensis (Turcz.) Baill, dried ripe fruits of Schisandraceae, was a famoustoxic traditional Chinense medicine. It′s main effective components were lignans andpolysaccharides compounds, which showed various positive biological effects such asantimicrobial, fatigue resistance, antitumor, anti-oxidation, anti-aging, anti-cancer, protectliver and the heart, regulate the immune system, inhibit the central nervous and otherpharmacological effect.In this study, taking S. chinensis as raw material and using the total extraction efficiencyof five lignans (SA, ST, DS, SB and SC) as index, and then analyzed by HPLC, the extractionconditions of STE was investigated systematically. Based on the investigation of single factor,which had a great effect on the extraction efficiency, RSM was designed to optimize theconditions. The result was75%ethanol as extraction solvent, solid-liquid ratio of1:19,sample sieved with120mesh, extraction voltage of180V and extraction time of1min. Underthese optimized conditions, the exraction efficiency of above five lignans in S. chinensiscollected from Baishan city had reached13.8139±0.0391mg/g. Compred with other methodsincluding RE, SE, MAE and UAE, STE not only had the highest extraction efficiency, butalso shortened exractiom time.S. chinensis polysccharides was extracted using the method of water extract-alcoholprecipitation, and then analyzed with phenol-sulphuric acid method. Taking the extractionefficiency as index, RSM was used to optimize the extraction conditions of MAEE. Theoptimal exraction process was as follows: microwave irradiation time of10min, extractionpH of4.21, extraction temperature of47.58℃, extraction timeof3h and enzymeconcentration of1.5%(wt.%of S. chinensis powder) for cellulase, papain and pectinase,respectively. Under these conditions, the CPS yield was7.38±0.21%. MAEE was then furthercompared with RE, UAE, MAE and EA. Result showed that MAEE had the highestextraction efficiency with lower temperature.The extraction conditions of lignans and polysaccharides in S. chinensis were studiedsimulateneously in this study, which provided the fundamental basisi for extraction ofbioactive components and promoted the use of S. chinensis resource.