Preparation And Application Of The Restricted Access Material Combined To Molecularly Imprinted Polymers Of2-methoxyestradiol

A restricted access material combined to molecularly imprinted polymer(RAM-MIP) has received much attention by analysts because the polymer offersmany advantages, such as its high selectivity, hydrophilicity and stability. As a result,it has great application potential in drug delivery system, food, pharmaceuticals andenvironmental monitoring analyses. In this study, the RAM-MIP was synthesized byprecipitation polymerization technique using17-βestradiol as the template withspecific recognition and selectivity for2-ME and at the same time, the polymeravoided the protein adsorption. Then the polymers were used as solid-phaseextraction (SPE) media to purify and enrich2-ME from complex matrix of thebiological samples, which can be regarded as an effective pretreatment procedurecontributing to effective detecting method. The main research works were as follows:1. Preparation and characterization of the restricted access material combined tomolecularly imprinted polymers of2-methoxyestradiol.The RAM-MIP was designed by precipitation polymerization and the impactionof template bleeding for trace analysis was avoided by the application of17β-estradiol as template. The synthesis condition was optimized. The polymer wascharacterized by fourier infrared spectrum, scanning electron microscope, nanoparticle sizer and thermo gravimetric analysis. The protein adsorption capacity andselectivity of2-ME were studied. Simultaneously, polymers’ characteristics werestudied by isothermal absorption methods and scatchard analysis. The prepared microspheres have good hydrophilicity, high specific reorganization ability andsignificantly reduced the irreversible adsorption of protein.2. A novel restricted access material combined to molecularly imprinted polymersfor selective solid-phase extraction and high performance liquid chromatographydetermination of2-methoxyestradiol in plasma samples.The obtained material was used as a selective matrix of SPE. Through the solidphase extraction-high performance liquid chromatography techniques, a detectionmethod which has specific recognition and high recovery rate of2-ME in plasmasamples was established. The results showed a good linearity range of0.06-20μg/ml. The LOD for2-ME standard were0.02μg/ml. The intra-assay precision was2.9%-9.3%. Inter-day precision was3.5%-11.9%. The recovery was99.10-101.00%.The proposed procedure is convenient, fast and specificity. It was applied for theroutine analysis of drugs in biological samples that obtained satisfactory results.3. Preparation and characterization of the restricted access matrix combined tomolecular imprinted monolithic columns of2-ME.In this study, monolithic columns for2-ME were prepared by the method ofin-suit polymerization, using restricted access matrix combined to molecularlyimprinted technology. The identification of2-ME and the adsorption capacity ofprotein were studied. The monolithic columns were applied for extraction andenrichment of2-ME in plasma samples. As a result, the monolithic column canremove the macromolecular protein in biological samples. At the same time, the drughas a good retention on the monolithic column. The proposed procedure is convenient,fast and specificity for the analysis of2-ME in biological samples.