Study On The Correlation Between Traumatic Brain Injury And The Expressions Of Serum MCP-1, VEGF, CD34~+Cells Of Damage Zone In Rats

Background:Traumatic brain injury is one of the most common diseases of Neurosurgery, itshigh mortality and morbidity in patients with serious harm to the health, strengthenits basic research is a major issue in neurosurgery. Light, the prognosis of severehead injury with a large difference in the severity of ischemic brain damage area isone of the reasons to change the prognosis differs significantly. Marshall found thatthe patient died of traumatic brain injury,90%had ischemic changes, ischemicchanges is the main mechanism of secondary injury. Monocyte chemotactic factor(Monocyte Chemoattractant Protein-1, MCP-1) is an important inflammatorychemokines, studies have shown that brain injury chemokines involved in thepathophysiology of the microcirculation of the important factors, but these indicatorschanges in the severity of the injury and the correlation is uncertain. Brain injury isthe area of neovascularization of ischemic brain tissue to improve the premise, thebasic function of synaptic connections of neurons is also reconstructed. VascularEndothelial Growth Factor (Vascular Endothelial Growth Factor, VEGF) is the mostimportant angiogenesis inducing factor. CD34+cells can promote the regenerationof the local capillary network, participate in the reconstruction of damaged bloodvessels. For this study, therefore through different levels of serum VEGF detected inrats after traumatic brain injury, CD34+cell changes in the expression of MCP-1expression levels and the damage zone, to study their degree of brain damagecorrelation.Objective:To explore the correlation between the degrees of traumatic brain injury and theexpressions of serum monocyte chemoattractant protein-1(MCP-1), serum vascularendothelial growth factor(VEGF), CD34+cells of damage zone in rats. Methods:Established the rat brain traumatic model according to Feeney protocol.Eightyclean adult SD rats were randomly divided into control group,sham-operatedgroup,the light injury group and the heavy injury group,40rats per group.Collectedthe peripheral blood respectively at1h,3h,6h,9h,1d,3d,5d,7d after the rats wereinjured. The expressions of serum MCP-1and VEGF were detected by ELISA.Affused the rats with paraformaldehyde and collected the brain tissue at6h,1d,3d,7dafter they were injured.The expressions of CD34+cells in the injury area weredetected by SP staining.Results:ELISA showed the expressions of MCP-1in peripheral blood were low incontrol group and sham-operated group but increased in light injury group and heavyinjury group at3h after injury,reached the peak at1d,and maintained this level until7d.The expressions of MCP-1of heavy injury group was higher than light injurygroup (P＜0.05). The expressions of VEGF in peripheral blood were extremely lowin control group and sham-operated group,but increased in light injury group andheavy injury group at6h after injury, and reached the peak at3d,and then maintainedthis level until7d.The expressions of VEGF of light injury group was higher thanheavy injury group (P＜0.05).Immunohistochemistry showed that the expressions ofCD34+cells of the damage zone were very low in control group and thesham-operated group.The CD34+cells appeared in injury area of light injury groupand heavy injury group,and the amount of CD34+cells in heavy injury group was lessthan light injury group.Conclusions:The expressions of serum MCP-1were positively correlated to the severity ofbrain trauma injury, the expressions of serum VEGF and the amount of CD34+cellsin the area of brain injury were negatively correlated to the severity of brain trauma injury,and all of this could be used to evaluate the severity of traumatic brain injury inrats.