Preparation Of Nano-Ag And Determination Of The Drug For Sers Method In The Study

Surface-enhanced Raman spectroscopy (SERS) since technology has been a concern since the discovery as an important modern detection technology, enabling the Raman signal of the analyte molecules has been a strong increase. SERS has a high sensitivity, low background fluorescence, non-destructive detection of samples, etc., so it has a wide range of applications in the chemical industry analysis, biological analysis and detection, and many other areas of medicine.Silver nanoparticles due to have some very special properties, such as surface effect, quantum size effect, so that the silver nanoparticles can be widely used in surface-enhanced Raman spectroscopy is one of the enhanced SERS substrate material works best. Preparation of silver nanoparticles has been a hot area of research in SERS applications. Traditional preparation of silver nanoparticles are generally more complex, influenced by many factors shaping difficult to control, thus impeding its wide application in the SERS. This article was prepared silver nanoparticles and their main focus in the determination of surface-enhanced Raman spectroscopy of organic small molecule drug application were discussed. Using a liposome and SDS-cyclohexane microemulsion method to prepare nano-silver particles, and use the resulting silver nanoparticles prepared SERS active substrate for the determination of acetaminophen.1、Combined with the development of new formulations of pharmaceutical preparations, to explore a new method of liposome preparation of silver nanoparticles, the research methods and reaction conditions for preparing silver nano particle size, morphology influence on, select the optimal prescription processes to Preparation of uniform size, performance and stability of nano-silver particles, and its characterization. In determining the final nano-Ag film dispersion method liposome select ether as organic solvent, soybean phospholipid to cholesterol ratio of3:1, to determine the concentration of silver nitrate solution is0.03M, other experimental conditions were the bath temperature is40℃, lemon sodium acid concentration of1%ultrasonic time20min. The final morphology of silver nanoparticles obtained mainly dominated spherical particle size controlled between20～80nm.2、Microemulsion with the traditional method of nano-silver particles were prepared by combining select SDS-cyclohexane microemulsion system to prepare nano-silver, sodium dodecyl sulfate (SDS) as a surfactant, co-surfactant that isopentanol active agent, a continuous oil phase cyclohexane solution of silver nitrate or hydrazine hydrate is dispersed to restore AgNO3by hydrazine hydrate to prepare uniform particle size, performance and stability of silver nanoparticles and its characterization. The results show that when W=5, the concentration of silver nitrate solution was prepared by microemulsion0.2M obtained when silver nanoparticles, can be obtained as spherical morphology of nanoparticles, the average particle size distribution in the range between50～100nm.3、Obtained using silver nanoparticles to prepare SERS active substrates, and the study drug molecules for applications in SERS, and by changes in the different SERS substrates to study the effects of various silver nanoparticles characterization of its activity. The results showed that the silver nanoparticles prepared by Method When the particle size of liposomes between20～80nm, reinforce the strength of a larger assembly time between24h-48h to give the activity of the substrate. SERS can be used to draw on the detection of acetaminophen, and can be qualitatively analyzed with simple operation, high sensitivity.