| Objective:To investigate the expression of y-aminobutyric acid transporter1(GAT-1) and glutamate transporter1(GLT-1) in immature hippocampus following recurrent seizures and the effects of progesterone on them, and discuss the relationship of GAT-1and GLT-1and infant epilepsy, meanwhile, to explore the anticonvulsant mechanisms of progesterone on developing rat.Methods:Seventy-two of postnatal (PN)7d Sprague-Dawley rats were randomly divided into three groups:the progesterone interventiongroup, the seizure group and the control group, each group had24rat pups. Seizures in rats were induced by inhalant flurothyl daily in six consecutive days.8of rat hippocampus were respectively sampled in each group at PN13d, PN15d, and PN19d. The expressions of GAT-1and GLT-1proteins in hippocampus were detected by immunohistochemistry and Western blot analysis.Results:Compared to the control group, the average optical density (AOD) of GLT-1and GAT-1immunoreactivity (IR) were increased significantly in the hippocampus of seizure group at all three time points (P<0.05). The AOD of GLT-1IR in the hippocampus of the progesterone intervention group was increased significantly than that in the control group and the seizure group at all three time points (P<0.05). while the AOD of GAT-1IR in the hippocampus of the progesterone intervention group was decreased significantly than that in the hippocampus of seizure group at all three time points, and increased significantly than that in the hippocampus of control group on PN13d (P＜0.05). Western blot showed that the GLT-1and GAT-1protein expressions in the hippocampuse of seizure group were increased significantly at all three time points than the control group at all three time points (P<0.05); the GLT-1protein expression in the hippocampus of progesterone intervention group increased significantly than that in the hippocampus of the control group and seizure group at all three time points (P<0.05); the GAT-1protein expression in the hippocampus of progesterone intervention group decreased significantly than that in the hippocampus of the seizure group and increased significantly than that in the hippocampus of the control group at all three time points (P<0.05).Conclusions:The abnormal expressions of GAT-1and GLT-1in immature brain after recurrent seizures may be involved in the pathology of developing brain injury. Progesterone can sustain the balance between the excited and inhibitory system in brain though the regulation of GAT-1and GLT-1expression after seizures, which would be one of the anticonvulsant mechanisms on developing brain injury. |
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