Impaired Adipogenesis In Adipose Tissue Associated With Hepatic Lipid Deposition Induced By Chronic Inflammation In Mice With Chew Diet

Objective: Chronic inflammation might be associated with hepaticlipid deposition independent of overnutrition. However, the mechanism isnot fully understood. In this study, we investigate if impaired adipogenesis inadipose tissue associated with hepatic lipid deposition induced by chronicinflammation in mice with chew diet.Methods:18male C57BL/6J mice were randomly divided into normalcontrol group (NC, n=9) and casein group (casein, n=9). Casein injection inC57BL/6J mice was given every other day to induce chronic inflammation.All mice were sacrificed after18weeks injections. Serum, liver and adiposetissue were collected for analysis. Real-time polymerase chain reaction andwestern blot were used to examine the gene and protein expressions ofmolecules involved in hepatic lipid metabolism and adipose adipogenesis.Results: Casein injection elevated the SAA mRNA levels of adiposetissue and liver, serum levels of insulin, FFA and proinflammatory factors.Hepatic lipid deposition was promoted by chronic inflammation. Chronic inflammation up-regulated the hepatic expression of CD36anddown-regulated MTP, CPT1a and ACOX1. Meanwhile, chronicinflammation not only minished the size of adipocytes, but alsodown-regulated the expressions of PPARγ and C/EBPα, both indicating animpaired adipogenesis.Conclusions: Besides disturbed lipid metabolism in the liver per se,impaired adipogenesis in the adipose tissue might also be associated withhepatic lipid deposition induced by chronic inflammation in mice with chewdiet.