Effect Of High Intensity Focused Ultrosound On Neuroglioma Zebrafish Model

Objective: Focused ultrosound(FU) is a new technology for thetreatment of tumor in recent years. FU kills the tumour cells chieflythrough the heating effect of the target area’s temperature. Theoretically,FU could treat the neuroglioma totally by means of decompressivecraniectomy (DC). This study aims to explore the treatment of FU onglioma zebrafish model, to observe the change of tumor tissue and vessels,and to investigate the effect of FU treatment on glioma and angiogenesis.Methods: Transgenic zebrafish (fli: GFP) were randomly divided intocontrol group, U87group and U87+HIFU group. Injecting neurogliomacells into2days post-fertilization zebrafish, after24h, the FU treatmentwas performed. The tumor size and the vessels were observed byfluorescence microscope at the1st day and the2nd day after the treatment.The mRNA expression of vascular endothelial growth factor (VEGF) Aaand vascular endothelial growth factor receptor (VEGFR)2were evaluatedby Real-time PCR. Results: In U87group, a large number of neuroglioma cells and thenewly formed vessels induced by neuroglioma were easily observed inzebrafish’s yolk ball, while after focused ultrosound treatment,neuroglioma cells and the vessels were significantly decreased. qPCRsuggested that the mRNA of VEGF Aa and VEGFR2were highlyexpressed in U87group (VEGF Aa：Fli:1.01±0.13vs. U87:1.45±0.10,P=0.002；VEGFR2：Fli:1.01±0.12vs. U87:1.41±0.07, P=0.001), butafter focused ultrosound treatment, the expression of VEGF Aa andVEGFR2were inhibited (VEGF Aa：U87+HIFU:1.14±0.05vs. U87:1.45±0.10, P=0.009；VEGFR2：U87+HIFU:1.16±0.06vs. U87:1.41±0.07,P=0.012).Conclusions: FU treatment can effectively kills the glioma cells,decreases the tumor angiogenesis and shows a positive treat effect onneuroglioma.