Study On The New Detection Methods Of The Gamma Ray Radiation Dose Based On The Spectra And Chromatography Characteristics Of Three Dyes

Gamma ray is a kind of electromagnetic wave with very short wavelength, whosepenetration is stronger than x-ray, also has the ability of kill cells. When the humanbody was irradiated by gamma, gamma rays can ingress into human body to causefragmentation of DNA in cells, even lead to death of cells when the situation is serious,and finally damage radiation. In recent years, radiation processing as a new technologydevelops rapidly，it has been widely applied in industry, agriculture, environmentalprotection, material science, medical and health care, and many other fields.Meanwhile, new detection methods for radiation dose have also become a hotspot athome and abroad. The dictation of gamma radiation dose has become a very importanttask for radiation protection, meanwhile, it is meaningful to study and establish a newmethod for gamma radiation dose detection. This thesis aims to study the new detectionmethods of the gamma ray radiation dose for the spectra and chromatographycharacteristics of three dyes.In the chapter1, the significance and current research progress of the healthlaboratory technologies for gamma-ray radiation dose was summarized, the maincontent and innovation of the health laboratory technologies also introduced briefly inour study.In the chapter2, we have developed a new detection method for thedetermination of60Co gamma radiation dose based on the fluorescence spectrumcharacteristic of acridine red dye. We use60Co gamma ray radiation AR solution toestablish the quantitative relationship D-F between gamma-ray radiation dose (D)and fluorescence intensity (F) according to the measured fluorescence intensities (F)of AR solution finally to establish the quantitative relationship D-F between gamma-ray radiation dose (D) and fluorescence intensity (F). With the increase of60Co gamma radiation dose, fluorescence intensity (F) of acridine red (AR) willdecrease. When λex=530nm, λem=550nm, the concentration is1.0×10-5~1.0×10-4mol/L of AR solution, if the concentration is larger, the radiation dose range ofquantitative determination would be wider (0~4kGy), and the linear relationshipbetween radiation dose and fluorescence intensities is much better（r=0.9996）.Researches suggest that coexist substances similar with AR structure, acidity ofsolution and the radiation temperature have little impact on detection result. Ourstudy mainly explains the influence mechanism of H2O2, we have also verified themechanism of the gamma ray radiation caused by AR fluorescence throughspectroscopy and mass spectrometry. This method greatly reduced the damage of60Co gamma ray to human body when the radiation dose is measured by physicalmethods, which has the advantage in measuring cumulative doses.In the chapter3, we respectively apply spectrum and chromatogram technology toestablish the new methods of60Co gamma radiation dose detection. After60Co gammaradiation, the fluorescence intensity of the Pynonin Y solution will be changed sharplyand the fluorescence intensity value will be declined. At λex=548nm, λem=565nm,when Pynonin Y solution concentration c=5.0×10-5~1.0×10-4mol/L, the greaterPynonin Y solution concentration is, the wider range of radiation dosage (0~4kGy)under quantitative determination will be, the linear quantitative relationship betweenradiation dose and the fluorescence intensity will be much better (r=0.9992).Researches suggest that the co-existence material which is similar to the materialstructure of Pynonin Y, thus, the acidity of solution and the radiation temperature haslittle impact on detection results; processed nitrogen prompting the fluorescenceintensity value of Pynonin Y solution will be reduced. The study also expounds theinfluence mechanism of H2O2, which respectively apply spectrum, mass spectrum andnuclear magnetic resonance hydrogen spectrum technology to verify the mechanism ofgamma ray radiation caused by PynoninY fluorescence.In the chapter4, we create new measurement method of gamma radiation ofmethyl orange (MO) dye chromatographic spectrum to discuss the mechanism of gamma radiation degradation methyl orange dye. Taking60Co gamma ray as radiationsources to irradiate azo dye methyl orange aqueous solution, when gamma-rayradiation dose increases, absorbance value of methyl orange solution will decrease. Atwavelength of464nm, the concentration of methyl orange solutionc=5.0×10-5~1.0×10-4mol/L, the range of0~1.5kGy radiation dose can be quantitativemeasured; there is a good linear relationship between radiation dose and absorbancevalue (r=0.998). Experiment also discuss the conditions which will have impact onthe radiation degradation of initial concentration, radiation dose, the amount of H2O2,nitrogen treatment, acid or alkaline of solution which respectively apply spectroscopy,chromatography, hydrogen spectrum and mass spectrometry technology to verify themechanism of the gamma-ray radiation degradation of methyl orange dyede-colorization.