Low-dose UVB Radiation Regulate Apoptosis And Proliferation Of HaCaT By AKT Pathways

Objective: To study how low-intensity ultraviolet UVB (UltravioletB,280-315nm) irradiation affect on HaCaT cells and the potentialmechanism of HaCaT cell apoptosis and proliferation pathways and AKTsignaling pathway interfered.Methods: The experiments by pre-selection of the most suitable5mJ/cm2intensity of irradiated on HaCaT cells from18,36and72hours, thecells were collected for the detection of apoptosis and proliferation; The totalprotein in HaCaT cells were extracted from irradiation cell for western blotto detect the expression of AKT, Bad and the Bad-p136.Results: The apoptotic effects of5mJ/cm2intensity irradiation have nodistinguish among different time point and with control HaCaT cells. But theproliferation rate after72hours irradiation was significant higher thancontrol HaCaT cells. Immunoblotting experiments confirmed the expressionlevels of AKT and Bad-p136were significant increased compared to normalexpression of Bad protein after UVB irradiation.Conclusion: The long time5mJ/cm2intensity UVB radiation maypromote cell proliferation signaling pathway by AKT, which may be a potential risk factor for the development of skin tumors.