Studies On The Quality Standard Of Hyssopus Cuspidatus Boriss And Its Adulterant Nepeta Bracteata Benth

Objectives:1) Based on the Chinese Pharmacopoeia(2010), to establish the quality evaluation of H.cuspidatusand and its adulterant N.bracteata;2) To preliminary test the antioxidant activity of H.cuspidatus and its adulterant N.bracteata. Method:1) H.cuspidayus and N.bracteata were identified with pharmacognosy methods in terms of macroscopical characteristics, microscopical characteristics, physico-chemical identification, et al;2) A HPLC method was established for the determination of the oleanolic acid and ursolic acid contents in H.cuspidatus and its adulterants N. bracteata;3) A HPLC fingerprint of H.cuspidatus and its adulterant N.bracteata was established by similarity evaluation, cluster analysis and principal component analysis;4) The antioxidant activities of H.cuspidatus and N.bracteata were evaluated by different antioxidant tests, including free radical scavenging ability, pyrogallol autoxidation and iron ion reduction. Results:1) The stem of H.cuspidatus was not as hollow as N. bracteata s. There were several layers of round collenchyma cells spreading inside the epidermis of H.cuspidatus, which were not found in N.bracteata. The xylem of H.cuspidatus was fan-shaped arrangement, while the xylem of N.bracteata was wide. In the Keller-kiliani reaction, only the result of N. bracteata was positive;2) According to the test results of10batches of H.cuspidatus, the moisture shall not be over9%, the total ash content shall not be over16%, the acid insoluble ash content shall not be over6%, the heavy metals shall not be more than3ppm, the arsenic salt shall not be more than1ppm;the corresponding results of N. bracteata were as follow:the moisture shall not be over8%, the total ash content shall not be over20%, acid insoluble ash content shall not be over7%;3) The average contents of oleanolic acid and ursolic acid of H. cuspidatus were higher than those of N. bracteata;4) The similarity analysis of the HPLC fingerprint showed that there were not significant difference between H.cuspidatus and its adulterants N.bracteata, and the different original H.cuspidatus and its adulterants N.bracteata could be divided into two categories;5) The different extracts of95%ethanol extracts from H.cuspidatus and N.bracteata showed varying degrees of antioxidant activity in vitro. Conclusion:1) According to the pharmacognosy identification of H.cuspidatus and N.bracteata, the identification bases will be provided for H.cuspidatus and its adulterants;2) The firstly-established HPLC fingerprint of H.cuspidatus and its adulterants N.bracteata can provide experimental bases on the quality control and evaluation for the different areas’s herb materials;3) According to the multi-index of the Quality evaluation, the quality standard draft of H.cuspidatus has been established, which will provide the basis for evaluating the qualities of H.cuspidatus and its adulterants N.bracteata;4) H.cuspidatus and its adulterants N.bracteata also have antioxidant activities, which will provide a basis for the further pharmacodynamics research of H.cuspidatus and N.bracteata.