Effect Of Abnormal Savda Munziq On The Oxidative Stress Response Of Parkinson’s Disease Rat Models

Objective:To observe the effects of Abnormal Savda Munziq(ASMq) on the behavior and oxidative stress response of Parkinson’s disease(PD) rat models.Methods: PD rats were produced by stereotactic injection of6-OHDA into the substantia nigra compacta(SNc) and the midbrain ventral tegmental area(VTA) of the right side of the brain.We set up regular blank.control group and the PD models were randomly divided into the model blank control group、the madopar group(madopar125mg/kg), and the low、 middle and high dose of ASMq group(1g/kg、3g/kg、6g/kg), and each group had10rats.The behavior of each group rats were observed before and after40days’ treatment.After the treatment each group getted randomly2rats for the immunohistochemical detection for the quantity change of tyrosine hydroxylase(TH) expression.The activity of superoxidase dismutase(SOD)、glutathione peroxidase(GSH-Px) and malonaldehyde(MDA) of the substantia nigra of the rest8rats of each group.Results:Compared with the model blank control group,the number of rotation in low dose of ASMq group was (12.50±1.50)(r/min),it was not signinficantly decreased,there was no statistical significance(P>0.05),but the number of rotation was respectively (6.00±1.07)、(10.38±1.69)、(7.00±1.31) in the madopar group、middle and high dose of ASMq group,they were decreased signinficantly,the data had ststistical significance (P<0.01). Compared with the model blank control group,the TH expression of low dose of ASMq group was (9.13±2.23), the increase was not significant, there was no ststistical significance(P>0.05),but the TH expression of the madopar group、middle and high dose of ASMq group was respectively (50.38±2.33)、(25.36±2.56)、(48.13±4.00), they were increased signinficantly,the data had ststistical signifycance (PM0.01). Compared with the model blank control group, the content of SOD and GSH-PX in low dose of ASMq group was (111.74±6.22) U/mg and (177.95±4.59) U/mg, the increase was not significant, the content of MDA was (7.02±0.40) nmol/ml,the decrease was not significant,there was no statistical significance(P>0.05). The content of SOD in the madopar group、middle and high dose of ASMq group were respectively (146.82±6.19)、(123.27±5.74)).(140.48±11.05) U/mg, The content of GSPH-PX were respectively (222.92±4.83).(185.46±8.20)、(219.03±2.66) U/mg,they were increased signinficantly,the content of MDA was respectively(4.89±0.24)、(6.12Q0.29),(5.21±0.38) nmol/ml,they were decreased signinficantly,all the data had statistical significance (P<0.01).Compared with the madopar group,the number of rotation、the TH expression and the content of SOD, GSH-Px and MDA in high dose of ASMq group,there was no statistical significance(P>0.05),although the above mentioned indicators in middle dose of ASMq group had improved significantly,there was no statistical significance(P>0.05). Compared with the high dose of ASMq group, the above mentioned indicators in middle dose of ASMq group,all the data had statistical significance (P<0.01)Conclusion:The high and middle dose of ASMq could signinficantly improve the behavior and maybe have the ability of anti-oxidation, the effects of high dose group were similar with the madopar group, which was superior to the middle and low dose of ASMq.