The Application Prospect Of Dinucleotide STR In Paternity Testing

Objective:To investigate the application prospect of dinucleotide STR selected in paternity testing. Methods:Dinucleotide STR loci D6S261、D15S216were selected,200unrelated blood samples from Shanghai were collected,to investigate the allele frequency and statistical parameters for forensic idengtification.and193paternity testing blood samples including58normal samples、9mutation samples were collected to investigate the application of dinucleotide STR in paternity testing.The touch-down PCR condition was used,and declined the denaturalization temperature to85℃,then amplified.the PCR products were collected by3130x1Gene-tic Analyzer,and analyzed with PowerStatsv12.xls. Results:15alleles,50genotype and H、DP、PE、PIC were found from200unrelated blood samples of D6S261;mutation were not found in193paternity testing blood samples;Only6alleles were found in D15S216,then delected D15S216.Conclusion:D6S261is highly polymorphic, decrease more stutter bands, the characteristic of dinucleotide STR with low mut-ation rate and high stab-ility,is a effective method to solve the indetermination of paternity testing caused by mutation.