Construction Of PDLLA Bone Tissue Engineering Scaffolds And Study Of Biological Properties In Vitro

The complications of large bone defects caused by various reasonsnot only increase the pains and treatment costs of patients, but also reducethe quality of patients’ life, so bone grafts as a conventional treatment iscommon and vital. At present, the conventional methods of bone graftssuch as autologous bone graft, allograft and artificial bone transplantation,is difficult to meet the clinical needs because they have some problemsand limitations.The research of bone defect treatment has become one ofthe hot research topic in the field of orthopedics, but now it is lack of idealtreatment method.In the1980s the rise of tissue engineering provides a new thought andmethod for bone defect repair. With the application of cell biology andengineering principles, tissue engineering is used to be the biologicalsubstitutes in vitro by building the damaged tissues.In general, there are three core factors in bone tissue engineering suchas seed cells、 biological scaffold and cell factor.It is a new milestone in the history of the development of life science following the cell biologyand molecular biology, marking the medicine will be out of the categoryof organ transplantation and step into a new era of human tissues andorgans.In recent years, bone tissue engineering have made great progress inthe fields of seed cells、cytokines and scaffold materials and successfullycultured bone tissue which has opened up new avenues for repair of largebone defects.But there is a large distance from the clinical application. Atpresent, How to build a good performance of scaffold materials aretwo key problems in bone tissue engineering field.Objective: To investigate the cytocompatibility of poly–D,L-lacticacid(PDLLA) in vitro and discuss the feasibility of PDLLA as bonetissue engineering scaffold materials.Methods:1.To isolate and identify the BMSCs using red blood cell crackingcombined with whole bone marrow adherent method in vitro, andexpansion to third generation to use as seed cells.2.To prepare the PDLLA three-dimensional porous scaffolds usingsolvent casting/particle drain analysis technology and observe itsbiological safety and cell compatibility.Results:1.The first generation of BMSCs was long spindle on the form and express CD29, CD90-positive, whereas expresse CD45-negative; Afterthree weeks of osteogenesis induction, BMSCs have been inducted forosteogenesis cells.2.The PDLLA scaffolds exhibited no significant negative influenceson the growth and osteogenesis differentiation of BMSCs and grown wellon the surface of the PDLLA scaffolds.Conclusion:1.The BMSCs in vitro can be successfully separated andaugmented,having good performance of growth and differentiation andcould be used as bone tissue engineering seed cells.2.PDLLA scaffolds has good performance of cell compatibility, andcould be used as a selection of tissue engineering scaffold materialsfor subsequent research.