Effects Of TSG On The Rat Carotid Artery Balloon-injury Model Smooth Muscle Cell Proliferation And PTEN Expression

Aims:To evaluate the effect of TSG on the inhibition of vascular smooth muscleproliferation and intimal hyperplasia on rat carotid artery after balloon injury and therelationship of PTEN expression.Methods:To choose30male SD rats(350~400g) from the Animal Center of the NanhuaUniversity, randomly divided them into the sham-operated group, the injury groupand the TSG group with10in each group, and the model of left carotid balloon injuryare established in the later two groups. After fourteen days, take the left carotid artery.A part of tissues are used to HE staining, observed the intimal proliferation and theVSMC proliferation by optical microscope. Other tissues are taken to detect theexpression of PTEN by the method of immunohistochemical staining and WesternBlot.Results:1. HE staining light microscopy and intimal hyperplasia in vascular smoothmuscle, the results showed that: after fourteen days, sham carotid intimal layer ofnon-proliferation of smooth muscle and performance, no neointimal formation;compared with the sham group,the surgery group and TSG group were vascularsmooth muscle proliferation and intimal layer, wall thickening, luminal narrowing,indicating that after balloon injury of vascular smooth muscle cells and intimalhyperplasia; while compared with the surgery group, TSG group of vascular smoothmuscle and proliferative changes in the intimal layer of light-operated group; HEstaining results suggest that TSG in vascular balloon injury rats can reduce smoothmuscle proliferation and intimal hyperplasia.2. The expression of PTEN in immunohistochemical and Western Blot detection of vascular tissue, the test results showed the three methods: Compared with shamgroup, the various time points after surgery, PTEN expression was significantlyhigher than surgical group (p <0.05), description vascular after balloon injury canmake PTEN expression was significantly increased; while compared with the surgerygroup, PTEN expression was significantly higher than TSG surgery group (p <0.05);description of TSG can upregulate the expression of PTEN.Conclusion:1. TSG significantly inhibit smooth muscle cell proliferation and intimalproliferation of the arteries after balloon injury;2. The mechanism may be related to its inhibitory effect on the expression ofPTEN.